Metabolomic insights into the effect of chickpea protein hydrolysate on the freeze–thaw tolerance of industrial yeasts

• Published in: Food chemistry 2024-05, Vol.439, p.138143-138143, Article 138143
• Main Authors: Kang, Sini, Xu, Yang, Kang, Yanyang, Rao, Junhui, Xiang, Fuwen, Ku, Seockmo, Li, Wei, Liu, Zhijie, Guo, Yaqing, Xu, Jianhua, Zhu, Xiangwei, Zhou, Mengzhou
• Format: Article
• Language: English
• Subjects: Bread - analysis; Chickpea protein hydrolysate; Cicer - metabolism; Fermentation; Freeze–thaw cycles; Freezing; Frozen dough; Metabolomics; Protein Hydrolysates - metabolism; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - metabolism; Yeast
• ISSN: 0308-8146; 1873-7072
• DOI: 10.1016/j.foodchem.2023.138143

[Display omitted] •Chickpea protein hydrolysate optimized the quality of frozen dough.•Chickpea protein hydrolysate enhanced viability of yeasts under freeze–thaw stress.•Metabolomics reveals a role for alterations of yeast intracellular metabolites.•Chickpea protein hydrolysate is a novel cryoprotectant for industrial yeasts. The use of frozen dough is an intensive food-processing practice that contributes to the development of chain operations in the bakery industry. However, the fermentation activity of yeasts in frozen dough can be severely damaged by freeze–thaw stress, thereby degrading the final bread quality. In this study, chickpea protein hydrolysate significantly improved the quality of steamed bread made from frozen dough while enhancing the yeast survival rate and maintaining yeast cell structural integrity under freeze–thaw stress. The mechanism underlying this protective role of chickpea protein hydrolysate was further investigated by untargeted metabolomics analysis, which suggested that chickpea protein hydrolysate altered the intracellular metabolites associated with central carbon metabolism, amino acid synthesis, and lipid metabolism to improve yeast cell freeze–thaw tolerance. Therefore, chickpea protein hydrolysate is a promising natural antifreeze component for yeast cryopreservation in the frozen dough industry.