Mobilization in two-step capillary isoelectric focusing: Concepts assessed by computer simulation

The mobilization step in a two-step capillary isoelectric focusing protocol is discussed by means of dynamic computer simulation data for systems without and with spacer compounds that establish their zones at the beginning and end of the focusing column. After focusing in an electroosmosis-free env...

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Bibliographic Details
Published in:Electrophoresis 2024-04, Vol.45 (7-8), p.618-638
Main Authors: Thormann, Wolfgang, Mosher, Richard A
Format: Article
Language:English
Subjects:
Capillary Isoelectric Focusing - methods
Computer Simulation
Electroosmosis
Electrophoresis, Capillary - methods
Hydrodynamics
Isoelectric focusing
Simulation
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Summary:The mobilization step in a two-step capillary isoelectric focusing protocol is discussed by means of dynamic computer simulation data for systems without and with spacer compounds that establish their zones at the beginning and end of the focusing column. After focusing in an electroosmosis-free environment (first step), mobilization (second step) can be induced electrophoretically, by the application of a hydrodynamic flow, or by a combination of both means. Dynamic simulations provide insight into the complexity of the various modes of electrophoretic mobilization and dispersion associated with hydrodynamic mobilization. The data are discussed together with the relevant literature.
ISSN:0173-0835
1522-2683
DOI:10.1002/elps.202300218