Tembusu virus induced apoptosis in vacuolate spermatogenic cells is mediated by Cytc-mediated mitochondrial apoptotic signaling pathway

Duck Tembusu virus (DTMUV) is an emerging mosquito-borne flavivirus that infects mainly poultry and has caused huge economic losses to the poultry farming industry in China. Also known as duck hemorrhagic ovarian disease, DTMUV principally destroys ovarian tissue in ducks, causing a dramatic drop in...

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Published in:Theriogenology 2024-02, Vol.215, p.312-320
Main Authors: Huang, Yufei, Zhang, Yafei, Yang, Sheng, Shi, Yonghong, Chu, Xiaoya, Ahmed, Nisar, Wu, Jingxian, Chen, Qiusheng
Format: Article
Language:English
Subjects:
Apoptosis
Cytochrome c
Duck tembusu virus
Spermatogenic cells
Testis
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Summary:Duck Tembusu virus (DTMUV) is an emerging mosquito-borne flavivirus that infects mainly poultry and has caused huge economic losses to the poultry farming industry in China. Also known as duck hemorrhagic ovarian disease, DTMUV principally destroys ovarian tissue in ducks, causing a dramatic drop in egg production. and can also invade the male reproductive system causing lesions. Currently, little research has been done to reveal the underlying mechanisms of reproductive dysfunction in ducks caused by DTMUV infection. In this study, histopathological analysis and electron microscopy of testes of ducks infected with DTMUV showed that DTMUV caused testicular atrophy and cytoplasmic vacuolation in ducks. Terminal Deoxynucleotidyl Transferase-Mediated Nick-End Labeling (TUNEL) staining and real-time quantitative PCR(RT-qPCR) results further indicated that DTMUV induced spermatogenic cells apoptosis. After DTMUV infection, a large amount of cytochrome c(Cytc) was released from the mitochondrial matrix into the cytoplasm, activating downstream target proteins and causing apoptosis. To sum up, DTMUV induces spermatogenic cell apoptosis through the Cytc-induced mitochondrial apoptosis pathway, our study provides evidence for DTMUV infection-induced male reproductive disorders. •DTMUV infection led to vacuolation in spermatogenic cells.•DTMUV infection induced apoptosis in spermatogenic cells.•Apoptosis in spermatogenic cells is regulated by multiple apoptotic pathways.•DTMUV infection activates caspase family proteins and promotes the release of Cytc from mitochondria.
ISSN:0093-691X
1879-3231
DOI:10.1016/j.theriogenology.2023.12.014