Limosomes versus hyalurolimosomes loaded with piperine for management of skin cancer

• Published in: International journal of pharmaceutics 2024-01, Vol.650, p.123730-123730, Article 123730
• Main Authors: El-Zahaby, Sally A, Abdelhady, Sherien A, Ali, Mennatallah A, Younis, Sameh E, Elnaggar, Yosra S R
• Format: Article
• Language: English
• Subjects: Alkaloids; Benzodioxoles; Humans; Hyaluronic Acid; Liposomes; Particle Size; Skin; Skin Neoplasms - drug therapy
• ISSN: 0378-5173; 1873-3476
• DOI: 10.1016/j.ijpharm.2023.123730

Skin cancer is considered the fifth most commonly occurring cancer worldwide hampering both health and economy. Piperine had proven efficacy in fighting skin cancer cells. Unfortunately, this natural agent had limited ability to penetrate the skin. The aim of the current study was to formulate piperine-loaded limosomes and hyalurolimosomes incorporating limonene as an edge activator and hyaluronic acid as bioactive gelling agent for managing skin cancer. Titration method followed by homogenization was adopted to prepare the nanoliposomal formulations. Characterization involved size, & zeta potential measurements, examination using transmission electron microscope (TEM) and stability study. Biological evaluation of the antitumor activity of piperine nanoliposomal formulations against Ehrlich's (EAC) solid tumor was also performed. Drug loaded limosomes and hyalurolimosomes had particle size; 346.55 ± 8.55 & 372.70 ± 10.83 nm, respectively. Zeta potential was high enough to ensure their stability. TEM micrographs detected the surrounding layer of Hyaluronic acid formed around the spherical limosomal nano-carrier ensuring the formation of Hyalurolimosomes. All stored formulations showed non-significant differences compared with freshly prepared ones at p