Interaction between membrane curvature sensitive factors SpoVM and SpoIVA in Bicelle condition

SpoVM and SpoIVA are essential proteins for coat assembly in Bacillus subtilis. SpoVM is a membrane curvature sensor, specifically localized on the forespore membrane. SpoIVA is an ATP hydrolase that self-assembles by hydrolyzing ATP. In this work, SpoVM and its mutant SpoVM were obtained by cyanoge...

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Published in:Biochemical and biophysical research communications 2024-01, Vol.694, p.149395, Article 149395
Main Authors: Chen, Jiali, Wang, Yifan, Lin, Shuru, Yu, Quanxiang, Qi, Zhengfei, Jiang, Wenqi, Zhao, Qiang, Fu, Qingshan Bill
Format: Article
Language:English
Subjects:
Adenosine Triphosphate - metabolism
Bacillus subtilis - metabolism
Bacterial Proteins - metabolism
Green Fluorescent Proteins - metabolism
Spores, Bacterial - metabolism
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container_title Biochemical and biophysical research communications
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creator Chen, Jiali
Wang, Yifan
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description SpoVM and SpoIVA are essential proteins for coat assembly in Bacillus subtilis. SpoVM is a membrane curvature sensor, specifically localized on the forespore membrane. SpoIVA is an ATP hydrolase that self-assembles by hydrolyzing ATP. In this work, SpoVM and its mutant SpoVM were obtained by cyanogen bromide cleavage and reconstituted into bicelles. The purification of SpoIVA was achieved through a rigorous process involving Ni-NTA chromatography column and size exclusion chromatography. This study utilized Biacore to obtain a direct determination of the kinetic parameters of interaction between SpoVM (SpoVM ) and SpoIVA in Bicelle conditions.
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subjects Adenosine Triphosphate - metabolism
Bacillus subtilis - metabolism
Bacterial Proteins - metabolism
Green Fluorescent Proteins - metabolism
Spores, Bacterial - metabolism
title Interaction between membrane curvature sensitive factors SpoVM and SpoIVA in Bicelle condition
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