Responses of photosynthesis-related genes in Sargassum horneri to high temperature stress

• Published in: Marine pollution bulletin 2024-02, Vol.199, p.115944-115944, Article 115944
• Main Authors: Dai, Wei, Wang, Xiaoran, Zhuang, Minmin, Sun, Jingyi, Shen, Yifei, Xia, Zhangyi, Wu, Tingjian, Jiang, Ruitong, Li, Aiqin, Bi, Fangling, Zhang, Jianheng, He, Peimin
• Format: Article
• Language: English
• Subjects: High temperature stress; Photosynthesis; Sargassum horneri; Transcriptome
• ISSN: 0025-326X; 1879-3363
• DOI: 10.1016/j.marpolbul.2023.115944

Golden tide outbreak threatened the marine ecological environment. Sargassum horneri is a single dominant species of the Yellow Sea golden tide, which growth and development are affected by changes in sea water temperature. This study investigated the photosynthetic physiology of copper algae and found that the growth rate, chlorophyll a content, carotenoid content, Fv/Fm, and maximum electron transfer efficiency were significantly reduced, indicating that Sargassum horneri was under stress under high temperature. In this study, high-throughput sequencing was used to analyze the response mechanisms of photosynthesis-related genes in S. horneri under high temperature stress. The results showed that most of the photosynthesis-related genes in S. horneri were downregulated and photosynthesis was inhibited under high temperature stress. However, the expression levels of ferredoxin, ferredoxin-NADP reductase, light-harvesting protein complexes, and oxygen-evolving complex genes were significantly upregulated (P ≤ 0.05) after five days of high temperature treatment. This study found that photosynthesis related genes play a crucial role in regulating the photosynthetic response of S. horneri to high temperature stress. •Under high temperature of 25 ℃, the light and parameters of Sargassum horneri decrease, indicating that they are under stress.•In this paper, ACT gene of Sargassum horneri was used as internal reference gene.•Under high temperature stress, the overall expression of genes related to the photosynthetic system of Sargassum horneri was limited.•The expression of OEC,LHC,B6/F genes in photosystem II of Sargassum horneri was down-regulated under short-term high temperature stress.•The upregulating number of PsaD,PsaE and LHC genes increased after 5 days of high temperature stress.