Effect of thermal shock and sustained heat treatment on mainstream partial nitrification and microbial community in sequencing batch reactors

• Published in: Environmental science and pollution research international 2024-01, Vol.31 (4), p.6258-6276
• Main Authors: Afroze, Niema, Kim, Mingu, Chowdhury, Mohammad M. I., Haroun, Basem, Andalib, Mehran, Umble, Arthur, Nakhla, George
• Format: Article
• Language: English
• Subjects: Accumulation; Activated sludge; Ammonia; Aquatic Pollution; Atmospheric Protection/Air Quality Control/Air Pollution; Bacteria; Batch reactors; Biomass; Bioreactors; Earth and Environmental Science; Ecotoxicology; Environment; Environmental Chemistry; Environmental Health; Heat; Heat treatment; Heat treatments; Heating; High temperature; Load distribution; Loading rate; Low temperature; Microorganisms; Municipal wastewater; Nitrification; Nitrifying bacteria; Nitrites; Nitrogen removal; Reactors; Research Article; Retention time; Sequences; Sequencing batch reactor; Thermal shock; Waste Water Technology; Water Management; Water Pollution Control
• ISSN: 1614-7499; 0944-1344; 1614-7499
• DOI: 10.1007/s11356-023-31421-8

In order to develop a promising means of achieving mainstream short-cut nitrification, this study evaluated the effect of thermal shock on nitrite accumulation using intermittent offline and continuous inline heat treatment of biomass in sequencing batch reactors (SBRs). The SBRs fed with municipal wastewater were operated at a solid retention time of 7 days and nitrogen loading rate of 0.04 gN/L·d to 0.08 gN/L·d without the application of pre-treatment. Contrary to literature studies that showed suppression of nitrite-oxidizing bacteria at temperature 60 to 80 °C, nitrite accumulation was achieved temporarily when 20% of the biomass was heated for 2 h at 47 °C, as well as in continuously heated SBRs at 37 °C and 42 °C. The continuously heated reactors at 37 °C and 42 °C produced a maximum nitrite accumulation ratio (NAR) of 0.59 and 0.79, respectively, whereas the intermittent offline heating at 47 °C-2 h produced a NAR of 0.37. Although nitrite accumulation was stable only for 10–12 days in all heated reactors, this study demonstrates the achievement of mainstream partial nitrification (PN) at lower temperature (42 °C) than that reported in literature and also highlights the potential for achieving PN by implementing heat treatment of a portion of the return activated sludge (RAS) in biological nitrogen removal (BNR) systems. During the time when full nitrification was achieved, Nitrospira was more dominant than Nitrosomonas in all reactors at ratios of 1.4:1, 2.4:1, 2.4:1, and 3.7:1 for the control SBR (22 °C), 47 °C -2 h offline heating SBR, 37 °C SBR, and 42 °C SBR, respectively, suggesting that it may have played a role as a comammox bacteria capable of degrading ammonia to nitrates at elevated temperature.