Ratiometric luminescent sensor based on BSA-coated gold/silver nanoclusters for the selective determination and spatiotemporal imaging of gallic acid in plants

A new fluorescence sensing strategy has been developed. Four bimetallic nanoclusters, gold/silver, gold/copper, gold/molybdenum and gold/cobalt, were prepared using bovine serum albumin (BSA) as a reducing and stabilizing agent. The fluorescence properties of four nanoclusters were explored by solid...

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Published in:Mikrochimica acta (1966) 2024-01, Vol.191 (1), p.60-60, Article 60
Main Authors: Wang, Mengyuan, Liu, Zhixin, Wu, Meng, Wang, Tong, Yu, Xueling, Niu, Na, Chen, Ligang
Format: Article
Language:English
Subjects:
Analytical Chemistry
Bimetals
Biocompatibility
Characterization and Evaluation of Materials
Chemistry
Chemistry and Materials Science
Fluorescence
Gallic acid
Gallic Acid - analysis
Gold
Metal Nanoparticles
Microengineering
Nanochemistry
Nanoclusters
Nanotechnology
Optical Imaging
Original Paper
Plants - chemistry
Serum albumin
Serum Albumin, Bovine
Silver
Stabilizers (agents)
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Summary:A new fluorescence sensing strategy has been developed. Four bimetallic nanoclusters, gold/silver, gold/copper, gold/molybdenum and gold/cobalt, were prepared using bovine serum albumin (BSA) as a reducing and stabilizing agent. The fluorescence properties of four nanoclusters were explored by solid-state UV and XPS. The gold/silver nanoclusters (BSA-Au/Ag NCs) with the best ratiometric fluorescence properties for gallic acid (GA) in plants were selected to realize the sensitive detection of GA. GA affected the conformation of BSA, thereby disrupting the luminescent environment of the nanoclusters, resulting in a pronounced fluorescence quenching at 566 nm. The ratiometric fluorescence signal (I 566 /I 453 ) was used for trace detection of GA in plants. It has a wide response range of 1.25–40.0 μM and a low detection limit of 45.27 nM. GA was detected at 19.49 μM in the plant extract, and the spiked recoveries ranged from 96.09 to 104.6%. In addition, due to the non-toxic and biocompatible properties of BSA, BSA-Au/Ag NCs have also been validated for fluorescence imaging of plant tissues. It realized the comparison of GA content in different parts of plants and the difference of GA content in plants after abiotic stress. Therefore, the developed strategy offers potential application for the analytical study of active substances in plants. Graphical Abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-023-06156-5