Revealing viral diversity in the Napahai plateau wetland based on metagenomics

• Published in: Antonie van Leeuwenhoek 2024-12, Vol.117 (1), p.3, Article 3
• Main Authors: Xiong, Lingling, Li, Yanmei, Zeng, Kun, Wei, Yunlin, Li, Haiyan, Ji, Xiuling
• Format: Article
• Language: English
• Subjects: Amino acids; Aquatic ecosystems; Bacteriophages; Biogeochemical cycles; Biogeochemistry; Biomedical and Life Sciences; Biosynthesis; China; DNA biosynthesis; DNA viruses; Ecological function; Ecosystem; Life Sciences; Marine ecosystems; Marine environment; Medical Microbiology; Metabolic pathways; Metabolism; Metagenomics; Microbiology; Microorganisms; Original Paper; Pentose; Pentose phosphate pathway; Phages; Phylogeny; Plant Sciences; Soil Science & Conservation; Sulfur; Viruses; Wetlands
• ISSN: 0003-6072; 1572-9699; 1572-9699
• DOI: 10.1007/s10482-023-01912-2

We focused on exploring the diversity of viruses in the Napahai plateau wetland, a unique ecosystem located in Yunnan, China. While viruses in marine environments have been extensively studied for their influence on microbial metabolism and biogeochemical cycles, little is known about their composition and function in plateau wetlands. Metagenomic analysis was employed to investigate the viral diversity and biogeochemical impacts in the Napahai wetland. It revealed that the Caudoviricetes and Malgrandaviricetes class level was the most abundant viral category based on phylogenetic analysis. Additionally, a gene-sharing network highlighted the presence of numerous unexplored viruses and demonstrated their unique characteristics and significant variation within the viral community of the Napahai wetland. Furthermore, the study identified the auxiliary metabolic genes (AMGs). AMGs provide phages with additional functions, such as protection against host degradation and involvement in metabolic pathways, such as the pentose phosphate pathway and DNA biosynthesis. The viruses in the Napahai wetland were found to influence carbon, nitrogen, sulfur, and amino acid metabolism, indirectly contributing to biogeochemical cycling through these AMGs. Overall, the research sheds light on the diverse and unique viral communities in the Napahai plateau wetland and emphasizes the significant roles of viruses in microbial ecology. The findings contribute to a deeper understanding of the characteristics and ecological functions of viral communities in plateau wetland ecosystems.