Marsh sediments chronically exposed to nitrogen enrichment contain degraded organic matter that is less vulnerable to decomposition via nitrate reduction

• Published in: The Science of the total environment 2024-03, Vol.915, p.169681-169681, Article 169681
• Main Authors: Bulseco, Ashley N., Murphy, Anna E., Giblin, Anne E., Tucker, Jane, Sanderman, Jonathan, Bowen, Jennifer L.
• Format: Article
• Language: English
• Subjects: Carbon storage; Decomposition; Denitrification; DNRA; Microbes; Nutrient enrichment; Sulfate reduction
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.169681

Blue carbon habitats, including salt marshes, can sequester carbon at rates that are an order of magnitude greater than terrestrial forests. This ecosystem service may be under threat from nitrate (NO3−) enrichment, which can shift the microbial community and stimulate decomposition of organic matter. Despite efforts to mitigate nitrogen loading, salt marshes continue to experience chronic NO3− enrichment, however, the long-term consequence of this enrichment on carbon storage remains unclear. To investigate the effect of chronic NO3− exposure on salt marsh organic matter decomposition, we collected sediments from three sites across a range of prior NO3− exposure: a relatively pristine marsh, a marsh enriched to ~70 μmol L−1 NO3− in the flooding seawater for 13 years, and a marsh enriched between 100 and 1000 μmol L−1 for 40 years from wastewater treatment effluent. We collected sediments from 20 to 25 cm depth and determined that sediments from the most chronically enriched site had less bioavailable organic matter and a distinct assemblage of active microbial taxa compared to the other two sites. We also performed a controlled anaerobic decomposition experiment to test whether the legacy of NO3− exposure influenced the functional response to additional NO3−. We found significant changes to microbial community composition resulting from experimental NO3− addition. Experimental NO3− addition also increased microbial respiration in sediments collected from all sites. However, sediments from the most chronically enriched site exhibited the smallest increase, the lowest rates of total NO3− reduction by dissimilatory nitrate reduction to ammonium (DNRA), and the highest DNF:DNRA ratios. Our results suggest that chronic exposure to elevated NO3− may lead to residual pools of organic matter that are less biologically available for decomposition. Thus, it is important to consider the legacy of nutrient exposure when examining the carbon cycle of salt marsh sediments. [Display omitted] •Long-term consequences of N enrichment on carbon storage remains unclear•Experimental N addition increases respiration but least when chronically enriched•The active microbial community shifts in response to experimental nitrate exposure•Chronically enriched marsh sediments have the highest DNF:DNRA ratios•Chronic nitrogen exposure may lead to less biologically available organic matter