Predictive metabolomic signatures for safety assessment of three plastic nanoparticles using intestinal organoids

• Published in: The Science of the total environment 2024-02, Vol.913, p.169606-169606, Article 169606
• Main Authors: Xuan, Lihui, Luo, Jinhua, Qu, Can, Guo, Peiyu, Yi, Wensen, Yang, Jingjing, Yan, Yuhui, Guan, Hua, Zhou, Pingkun, Huang, Ruixue
• Format: Article
• Language: English
• Subjects: autophagy; environment; fatty acid metabolism; HCT116 cells; Intestinal organoids; intestines; membrane potential; metabolites; Metabolomics; mice; mitochondrial membrane; Nanoplastic particles; nanoplastics; Nanotoxicity; necroptosis; organoids; oxidative stress; pollution; safety assessment; species; toxicity
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.169606

Nanoplastic particles are pervasive environmental contaminants with potential health risks, while mouse intestinal organoids provide accurate in vitro models for studying these interactions. Metabolomics, especially through LC-MS, enables detailed cellular response studies, and there's a novel interest in comparing metabolic changes across nanoparticle species using gut organoids. This study used a mouse intestinal organoid combined with cell model to explore the differences in metabolites and toxicity mechanisms induced by exposure to three nanoplastics (PS, PTFE, and PMMA). The results showed that PS, PTFE, and PMMA exposure reduced mitochondrial membrane potential, intracellular ROS accumulation and oxidative stress, and inhibited the AKT/mTOR signaling pathway. Non-targeted metabolomics results confirmed that three types of nanoplastic particles regulate cellular status by regulating fatty acid metabolism, nucleotide metabolism, necroptosis and autophagy pathways. More importantly, these representative metabolites were further validated in model groups after mouse intestinal organoids and HCT116 cells were exposed to the respective NPs, indicating that organoid metabolomics results can be used to effectively predict toxicity. Untargeted metabolomics is sensitive enough to detect subtle metabolomic changes when functional cellular analysis shows no significant differences. Overall, our study reveals the underlying metabolic mechanism of NPs-induced intestinal organoid toxicity and provides new insights into the possible adverse consequences of NPs. [Display omitted] •The metabolic toxicity of PS-NPs, PMMA-NPs, and PTFE-NPs can be predicted using non-targeted metabolomics.•Intestinal organoids are powerful models for assessing the metabolic toxicity of nanoplastics in vitro.•Different types of nanoplastics cause different metabolic toxicity mechanisms in intestinal organoids.