Characterization of Priestia megaterium S1, a polymer degrading gut microbe isolated from the gut of Tenebrio molitor larvae fed on Styrofoam

This study reveals that Tenebrio molitor larvae are fed with two different feeds i.e., barley bran along with Styrofoam, and barley bran without Styrofoam, the survival percentage of mealworms shows 86 and 89%, respectively. Five isolates namely S1, S2, S3, S4, and S5 were isolated from the gut of S...

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Published in:Archives of microbiology 2024-01, Vol.206 (1), p.48-48, Article 48
Main Authors: Akash, Krishnamoorthi, Parthasarathi, Rengasamy, Elango, Rajavel, Bragadeeswaran, Subramanian
Format: Article
Language:English
Subjects:
Barley
Barley bran
Biochemical oxygen demand
Biochemistry
Biodegradation
Biomedical and Life Sciences
Biotechnology
Carbon
Carbon dioxide
Cell Biology
Ecology
Fourier transforms
Hydrophobicity
Infrared spectroscopy
Larvae
Life Sciences
Microbial Ecology
Microbiology
Microorganisms
Original Paper
Oxygen demand
Polymers
Priestia megaterium
rRNA 16S
Scanning electron microscopy
Styrofoam
Survival
Tenebrio molitor
Turbidity
Weight loss
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Summary:This study reveals that Tenebrio molitor larvae are fed with two different feeds i.e., barley bran along with Styrofoam, and barley bran without Styrofoam, the survival percentage of mealworms shows 86 and 89%, respectively. Five isolates namely S1, S2, S3, S4, and S5 were isolated from the gut of Styrofoam-feeding Tenebrio molitor larvae and tested for Hydrophobicity percentage, clear zone assay and turbidity measurement. S1 isolate showed best (turbidity percentage of 19.65%, 13.54% hydrophobicity percentage, and 37% zone of clearance) when compared to other isolates, respectively. 16S rRNA characterization of S1 isolate revealed that the isolate belongs to Priestia megaterium S1(ON024787). Biodegradation of PE and PS beads by Priestia megaterium S1 makes physical and structural changes over 180 days, after microbial adhesion to the beads. Growth parameters have shown that the Priestia megaterium S1 thrives more effectively in the pH (6.5), temperature (28 °C) and at 1.5% LDPE/HDPE/PS concentration there is maximum utilization of carbon and a high percentage survival rate. Significant colonization of the isolate after 30 days over beads of LDPE (52.47%), HDPE (49.26%), and PS (48.11%), respectively. Experimental data revealed that Priestia megaterium S1 have PE and PS beads degradation capacity, proven by weight loss studies, at 6th-month percentage weight loss of LDPE (36.1%), HDPE (31.9%), and PS (28.6%), the percentage loss of carbon and hydrogen shows higher when compared to control. One month Biological Oxygen Demand (BOD) showed that LDPE (7.4 mg/l), HDPE (7.2 mg/l), PS (6.7 mg/l), and simultaneous studies on CO 2 evolution over LDPE treatment is 5.05 g/l, HDPE (4.26 g/l), and PS (3.91 g/l), respectively. Fourier Transform Infrared Spectroscopy (FTIR) and Scanning Electron Microscope (SEM) prove the occurrence of biodegradation on the surface of beads. This work highlights that Priestia megaterium S1 plays a vital role in effectively degrading PE and PS beads. Graphical abstract
ISSN:0302-8933
1432-072X
DOI:10.1007/s00203-023-03785-5