Characterization of a novel endornavirus isolated from the phytopathogenic fungus Rhizoctonia solani

• Published in: Archives of virology 2024-01, Vol.169 (1), p.15-15, Article 15
• Main Authors: Li, Yangyi, Huang, Xingxue, Zhou, Guolin, Ye, Anhua, Deng, Yaohua, Shi, Lingfang, Zhang, Runhua
• Format: Article
• Language: English
• Subjects: 3' Untranslated regions; 5' Untranslated Regions; Amino acids; Annotated Sequence Record; Biomedical and Life Sciences; Biomedicine; DNA helicase; DNA-directed RNA polymerase; Fungi; Genomes; Infectious Diseases; Medical Microbiology; Methyltransferase; Nucleotide sequence; Nucleotides; Phylogenetics; Phylogeny; Phytopathogenic fungi; Rhizoctonia solani; RNA helicase; RNA polymerase; RNA-directed RNA polymerase; Virology
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-023-05915-2

Rhizoctonia solani endornavirus 8 (RsEV8) was isolated from strain XY175 of Rhizoctonia solani AG-1 IA . The full-length genome of RsEV8 is 16,147 nucleotides (nt) in length and contains a single open reading frame that encodes a large polyprotein of 5227 amino acids. The polyprotein contains four conserved domains: viral methyltransferase, putative DEAH box helicase, viral helicase, and RNA-dependent RNA polymerase (RdRp). RsEV8 has a shorter 3'-UTR (58 nt) and a longer 5'-UTR (404 nt). A multiple sequence alignment indicated that the RdRp of RsEV8 possesses eight typical RdRp motifs. According to a BLASTp analysis, RsEV8 shares 39.31% sequence identity with Rhizoctonia cerealis endornavirus-1084-7. Phylogenetic analysis demonstrated that RsEV8 clusters with members of the genus Betaendornavirus .