EtOH-LN cryoembedding workflow to minimize freezing artifact in frozen tissues: A pilot study in preparing tissues compatible with mass spectrometry-based spatial proteomics application

Coolant-assisted liquid nitrogen (LN) flash freezing of frozen tissues has been widely adopted to preserve tissue morphology for histopathological annotations in mass spectrometry-based spatial proteomics techniques. However, existing coolants pose health risks upon inhalation and are expensive. To...

Full description

Saved in:
Bibliographic Details
Published in:Cryobiology 2024-03, Vol.114, p.104843-104843, Article 104843
Main Authors: Tan, Yong Chiang, Mustangin, Muaatamarulain, Rosli, Nurwahyuna, Wan Ahmad Kammal, Wan Syahira Ellani, Md Isa, Nurismah, Low, Teck Yew, Lee, Pey Yee, Chellappan, Dinesh Kumar, Jarmin, Razman, Zuhdi, Zamri, Azman, Azlanudin, Ian, Chik, Yusof, Nursuhadah Mohamed, Lim, Lay Cheng
Format: Article
Language:English
Subjects:
Cryoembedding
Ethanol
Flash freezing
Freezing artifact
Frozen tissue
Histology
Liquid nitrogen
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Coolant-assisted liquid nitrogen (LN) flash freezing of frozen tissues has been widely adopted to preserve tissue morphology for histopathological annotations in mass spectrometry-based spatial proteomics techniques. However, existing coolants pose health risks upon inhalation and are expensive. To overcome this challenge, we present our pilot study by introducing the EtOH-LN workflow, which demonstrates the feasibility of using 95 % ethanol as a safer and easily accessible alternative to existing coolants for LN-based cryoembedding of frozen tissues. Our study reveals that both the EtOH-LN and LN-only cryoembedding workflows exhibit significantly reduced freezing artifacts compared to cryoembedding in cryostat (p 
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2023.104843