Ion-bombardment-driven surface modification of porous magnesium scaffolds: Enhancing biocompatibility and osteoimmunomodulation

• Published in: Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2024-02, Vol.234, p.113717-113717, Article 113717
• Main Authors: Posada, Viviana M., Ramírez, Juan, Civantos, Ana, Fernández-Morales, Patricia, Allain, Jean Paul
• Format: Article
• Language: English
• Subjects: Animals; Biocompatible Materials - pharmacology; Cellular materials; Corrosion; Corrosion resistance; Directed Plasma Nanosynthesis; Magnesium; Magnesium - chemistry; Magnesium - pharmacology; Mice; Nano-medicine; Nanotopography; Porosity; Tissue Engineering; Tissue Scaffolds - chemistry
• ISSN: 0927-7765; 1873-4367
• DOI: 10.1016/j.colsurfb.2023.113717

Porous Mg scaffolds are promising for bone repair but are limited by high corrosion rates and challenges in preserving coating integrity. We used Directed Plasma Nanosynthesis (DPNS) at 400 eV and a fluence of 1 × 1018 cm−2 to augment the bioactivity and corrosion resistance of porous Mg scaffolds, maintaining their overall material integrity. DPNS creates nanostructures that increase surface area, promote apatite nucleation, and enhance osseointegration, improving the bioactivity and corrosion resistance of porous Mg scaffolds without compromising their structure. Our findings indicate a decrease in surface roughness, with pre-irradiated samples having Rq = 60.4 ± 5.3 nm andRa = 48.2 ± 3.1 nm, and post-DPNS samples showing Rq = 36.9 ± 0.3 nm andRa = 28.6 ± 0.8 nm. This suggests changes in topography and wettability, corroborated by the increased water contact angles (CA) of 129.2 ± 3.2 degrees. The complexity of the solution influences the CA: DMEM results in a CA of 120.4 ± 0.1 degrees, while DMEM + SBF decreases it to 103.6 ± 0.5 degrees, in contrast to the complete spreading observed in non-irradiated samples. DPNS-treated scaffolds exhibit significantly reduced corrosion rates at 5.7 × 10−3 ± 3.8 × 10−4 mg/cm²/day, compared to the control's 2.3 × 10−2 ± 3.2 × 10−4 mg/cm²/day over 14 days (P  100°.•Plasma radiation controls Mg2+ release, significantly reducing corrosion rates in porous Mg.•Plasma treatment affects hBM-MSC and J774 cells, influencing macrophage behavior.•In DMEM, plasma treatment controls Mg2+ and OH- ion release, aiding Ca-phosphate layer formation.