Proficiently partitioning of bioactive peptide-ssDNA conjugates by microbead-assisted capillary electrophoresis (MACE)

Low-molecular drug discovery using DNA-encoded chemical library (DEL) is a powerful technology, although improving the partitioning efficiency of affinity ligands from DEL remains a challenge. Here, we assessed the usefulness of microbead-assisted capillary electrophoresis (MACE) for partitioning pe...

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Bibliographic Details
Published in:Analytical biochemistry 2024-04, Vol.687, p.115452-115452, Article 115452
Main Authors: Matsuo, Muneyuki, Wakui, Koji, Inami, Yuuki, Furukawa, Akihiro, Sato, Seiji, Yoshimoto, Keitaro
Format: Article
Language:English
Subjects:
DNA - chemistry
DNA, Single-Stranded
Electrophoresis, Capillary - methods
Microspheres
Oligonucleotides - chemistry
Peptides - chemistry
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Summary:Low-molecular drug discovery using DNA-encoded chemical library (DEL) is a powerful technology, although improving the partitioning efficiency of affinity ligands from DEL remains a challenge. Here, we assessed the usefulness of microbead-assisted capillary electrophoresis (MACE) for partitioning peptide-oligonucleotide conjugates (POCs), in which high selection pressure is applied because of different mobility of target-modified beads and POCs during CE. Despite their different charge characteristics, all POCs were well separated from the beads. When bead extraction was performed, the tagged DNA amplification was observed only in the couple of a ligand/target, suggesting proficiently specific partitioning of peptide ligands was accomplished using MACE.
ISSN:0003-2697
1096-0309
DOI:10.1016/j.ab.2023.115452