Municipal wastewater treatment plant showing a potential reservoir for clinically relevant MDR bacterial strains co-occurrence of ESBL genes and integron-integrase genes

• Published in: Journal of environmental management 2024-02, Vol.351, p.119938-119938, Article 119938
• Main Authors: Soni, Kuldeep, Kothamasi, David, Chandra, Ram
• Format: Article
• Language: English
• Subjects: Anti-Bacterial Agents - pharmacology; Antibiotic resistance genes; Antibiotic-resistance dissemination; Antibiotic-resistant bacteria; Bacillus; Bacteria; beta-Lactamases - genetics; Drug Resistance, Multiple; Drug Resistance, Multiple, Bacterial - genetics; Escherichia coli; Extended-spectrum beta-lactamase; Humans; Integrases; Integrons - genetics; Mobile genetic elements; Wastewater; Water Purification
• ISSN: 0301-4797; 1095-8630
• DOI: 10.1016/j.jenvman.2023.119938

Municipal wastewater treatment plants (MWWTPs) are a milieu for co-occurrence of multiple antibiotic resistance genes (ARGs). This facilitates mixing and genetic exchange; and promotes dissemination of multidrug resistance (MDR) to wastewater bacterial communities which is hazardous for the effluent receiving environment. This study investigated the co-occurrence of extended-spectrum beta-lactamase (ESBL) genes (blaTEM, blaCTX-M, blaSHV, blaOXA), and integron-integrase genes (intI1, intI2, intI3) in MDR bacteria isolated from the Bharwara MWWTP in Lucknow, India. Thirty-one MDR bacterial colonies resistant to three or more antibiotics were isolated from three treatment stages of this MWWTP. Six of these: Staphylococcus aureus, Serratia marcescens, Salmonella enterica, Shigella sonnei, Escherichia coli, and Bacillus sp. Had co-occurrence of ESBL and integron-integrase genes. These six isolates were examined for the occurrence of MDR efflux genes (qacA, acrB) and ARGs (aac(3)-1, qnrA1, tetA, vanA) and tested for resistance against 12 different antibiotics. The highest resistance was against penicillin-G (100%) and lowest for chloramphenicol (16.66%). Bacillus sp. Isolate BWKRC6 had the highest co-occurrence of antibiotic resistance-determining genes and was resistant to all the 12 antibiotics tested. The co-occurrence of ESBL, integron-integrase, antibiotic resistance-determining and MDR efflux genes in bacteria isolated from the Bharwara MWWTP indicates that the wastewaters of this treatment plant may have become a hotspot for MDR bacteria and may present human and environmental health hazards. Therefore, there is need for a rapid action to limit the spread of this threat. Public regulatory authorities must urgently implement measures to prevent MWWTPs becoming reservoirs for evolution of antibiotic resistance genes and development of antibiotic resistance. [Display omitted] •Prevalence of antibiotic resistance (AR) in a wastewater treatment plant was studied.•36.5% of the isolated bacteria were resistant to three or more antibiotics.•ESBL, integron-integrase, AR and efflux genes co-occurred in six bacterial strains.•AR gene distribution frequency correlated strongly with wastewater physico-chemistry.