Continuous cyclodextrin glucanotransferase production by free and immobilized cells of Bacillus circulans ATCC 21783 in bioreactors

Four systems for a continuous cyclodextrin glucanotransferase (CGTase) production by Bacillus circulans ATCC 21783 were studied: free cells in a stirred reactor; free cells in a bubble column reactor; agar-entrapped and membrane-immobilized cells in a bubble column reactor. The performance of the pr...

Full description

Saved in:
Bibliographic Details
Published in:Process biochemistry (1991) 2005-10, Vol.40 (10), p.3290-3295
Main Authors: Vassileva, Anna, Beschkov, Venko, Ivanova, Viara, Tonkova, Alexandra
Format: Article
Language:English
Subjects:
Bacillus circulans
Bioreactors
Continuous enzyme production
Cyclodextrin glucanotransferase
Cyclodextrins
Free and immobilized cells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
cited_by cdi_FETCH-LOGICAL-c408t-3d230ebac424e5dcd2320b6a27f9289dab4db017d841a5dafc7ed65bb0af1ed3
cites cdi_FETCH-LOGICAL-c408t-3d230ebac424e5dcd2320b6a27f9289dab4db017d841a5dafc7ed65bb0af1ed3
container_end_page 3295
container_issue 10
container_start_page 3290
container_title Process biochemistry (1991)
container_volume 40
creator Vassileva, Anna
Beschkov, Venko
Ivanova, Viara
Tonkova, Alexandra
description Four systems for a continuous cyclodextrin glucanotransferase (CGTase) production by Bacillus circulans ATCC 21783 were studied: free cells in a stirred reactor; free cells in a bubble column reactor; agar-entrapped and membrane-immobilized cells in a bubble column reactor. The performance of the process with free cells led to a higher enzyme level, specific CGTase activity and enzyme productivity compared to systems using immobilized cells. However, the residual enzyme activity (after approximately 300 h process) was from 96 to 97% for immobilized cells and from 78 to 86% for free cells. The four used systems provided a significantly higher CGTase yield (from 3.4-fold to 4.7-fold) and productivity (from 1.4-fold to 2.9-fold) in comparison to known strains of Bacillus applied for the same aim. The concomitant high specific CGTase activity indicated that the established cultural conditions provided the dominant synthesis of the active protein.
doi_str_mv 10.1016/j.procbio.2005.03.022
format article
fullrecord <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_29110694</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>S1359511305001534</els_id><sourcerecordid>28497207</sourcerecordid><originalsourceid>FETCH-LOGICAL-c408t-3d230ebac424e5dcd2320b6a27f9289dab4db017d841a5dafc7ed65bb0af1ed3</originalsourceid><addsrcrecordid>eNqFkUFP3DAQhSNUJCjwE5B84pZ0bCeb5IRoVNpKSFz2btnjCfLKa1M7qViu_eP1arlz8lh68828eVV1y6HhwDffds1rimhcbARA14BsQIiz6pIPvaylGIcvpZbdWHecy4vqa847AMk5h8vq3xTD4sIa18zwgD5aeluSC-zFr6hDXJIOeaakM7Eyxa64uBiYObA5ETEdLHP7fTTOu3eyDMn7zOLMvmt03h-hLuHqC4Q9bKeJCd4PkhV-WTeRxiWmfF2dz9pnuvl4r6rt44_t9Kt-ev75e3p4qrGFYamlFRLIaGxFS53F8hVgNlr08yiG0WrTWgO8t0PLdWf1jD3ZTWcM6JmTlVfV3QlbfPxZKS9q7_JxYR2o2FdiLBfZjO3nwqEdewF9EXYnIaaYc6JZvSa31-mgOKhjNGqnPqJRx2gUSFWiKX33pz4qbv86Siqjo4BkXSJclI3uE8J_HHqdtA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>28497207</pqid></control><display><type>article</type><title>Continuous cyclodextrin glucanotransferase production by free and immobilized cells of Bacillus circulans ATCC 21783 in bioreactors</title><source>Elsevier</source><creator>Vassileva, Anna ; Beschkov, Venko ; Ivanova, Viara ; Tonkova, Alexandra</creator><creatorcontrib>Vassileva, Anna ; Beschkov, Venko ; Ivanova, Viara ; Tonkova, Alexandra</creatorcontrib><description>Four systems for a continuous cyclodextrin glucanotransferase (CGTase) production by Bacillus circulans ATCC 21783 were studied: free cells in a stirred reactor; free cells in a bubble column reactor; agar-entrapped and membrane-immobilized cells in a bubble column reactor. The performance of the process with free cells led to a higher enzyme level, specific CGTase activity and enzyme productivity compared to systems using immobilized cells. However, the residual enzyme activity (after approximately 300 h process) was from 96 to 97% for immobilized cells and from 78 to 86% for free cells. The four used systems provided a significantly higher CGTase yield (from 3.4-fold to 4.7-fold) and productivity (from 1.4-fold to 2.9-fold) in comparison to known strains of Bacillus applied for the same aim. The concomitant high specific CGTase activity indicated that the established cultural conditions provided the dominant synthesis of the active protein.</description><identifier>ISSN: 1359-5113</identifier><identifier>EISSN: 1873-3298</identifier><identifier>DOI: 10.1016/j.procbio.2005.03.022</identifier><language>eng</language><publisher>Elsevier Ltd</publisher><subject>Bacillus circulans ; Bioreactors ; Continuous enzyme production ; Cyclodextrin glucanotransferase ; Cyclodextrins ; Free and immobilized cells</subject><ispartof>Process biochemistry (1991), 2005-10, Vol.40 (10), p.3290-3295</ispartof><rights>2005 Elsevier Ltd</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c408t-3d230ebac424e5dcd2320b6a27f9289dab4db017d841a5dafc7ed65bb0af1ed3</citedby><cites>FETCH-LOGICAL-c408t-3d230ebac424e5dcd2320b6a27f9289dab4db017d841a5dafc7ed65bb0af1ed3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids></links><search><creatorcontrib>Vassileva, Anna</creatorcontrib><creatorcontrib>Beschkov, Venko</creatorcontrib><creatorcontrib>Ivanova, Viara</creatorcontrib><creatorcontrib>Tonkova, Alexandra</creatorcontrib><title>Continuous cyclodextrin glucanotransferase production by free and immobilized cells of Bacillus circulans ATCC 21783 in bioreactors</title><title>Process biochemistry (1991)</title><description>Four systems for a continuous cyclodextrin glucanotransferase (CGTase) production by Bacillus circulans ATCC 21783 were studied: free cells in a stirred reactor; free cells in a bubble column reactor; agar-entrapped and membrane-immobilized cells in a bubble column reactor. The performance of the process with free cells led to a higher enzyme level, specific CGTase activity and enzyme productivity compared to systems using immobilized cells. However, the residual enzyme activity (after approximately 300 h process) was from 96 to 97% for immobilized cells and from 78 to 86% for free cells. The four used systems provided a significantly higher CGTase yield (from 3.4-fold to 4.7-fold) and productivity (from 1.4-fold to 2.9-fold) in comparison to known strains of Bacillus applied for the same aim. The concomitant high specific CGTase activity indicated that the established cultural conditions provided the dominant synthesis of the active protein.</description><subject>Bacillus circulans</subject><subject>Bioreactors</subject><subject>Continuous enzyme production</subject><subject>Cyclodextrin glucanotransferase</subject><subject>Cyclodextrins</subject><subject>Free and immobilized cells</subject><issn>1359-5113</issn><issn>1873-3298</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2005</creationdate><recordtype>article</recordtype><recordid>eNqFkUFP3DAQhSNUJCjwE5B84pZ0bCeb5IRoVNpKSFz2btnjCfLKa1M7qViu_eP1arlz8lh68828eVV1y6HhwDffds1rimhcbARA14BsQIiz6pIPvaylGIcvpZbdWHecy4vqa847AMk5h8vq3xTD4sIa18zwgD5aeluSC-zFr6hDXJIOeaakM7Eyxa64uBiYObA5ETEdLHP7fTTOu3eyDMn7zOLMvmt03h-hLuHqC4Q9bKeJCd4PkhV-WTeRxiWmfF2dz9pnuvl4r6rt44_t9Kt-ev75e3p4qrGFYamlFRLIaGxFS53F8hVgNlr08yiG0WrTWgO8t0PLdWf1jD3ZTWcM6JmTlVfV3QlbfPxZKS9q7_JxYR2o2FdiLBfZjO3nwqEdewF9EXYnIaaYc6JZvSa31-mgOKhjNGqnPqJRx2gUSFWiKX33pz4qbv86Siqjo4BkXSJclI3uE8J_HHqdtA</recordid><startdate>20051001</startdate><enddate>20051001</enddate><creator>Vassileva, Anna</creator><creator>Beschkov, Venko</creator><creator>Ivanova, Viara</creator><creator>Tonkova, Alexandra</creator><general>Elsevier Ltd</general><scope>AAYXX</scope><scope>CITATION</scope><scope>7TB</scope><scope>8FD</scope><scope>F28</scope><scope>FR3</scope><scope>7U5</scope><scope>L7M</scope></search><sort><creationdate>20051001</creationdate><title>Continuous cyclodextrin glucanotransferase production by free and immobilized cells of Bacillus circulans ATCC 21783 in bioreactors</title><author>Vassileva, Anna ; Beschkov, Venko ; Ivanova, Viara ; Tonkova, Alexandra</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c408t-3d230ebac424e5dcd2320b6a27f9289dab4db017d841a5dafc7ed65bb0af1ed3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2005</creationdate><topic>Bacillus circulans</topic><topic>Bioreactors</topic><topic>Continuous enzyme production</topic><topic>Cyclodextrin glucanotransferase</topic><topic>Cyclodextrins</topic><topic>Free and immobilized cells</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Vassileva, Anna</creatorcontrib><creatorcontrib>Beschkov, Venko</creatorcontrib><creatorcontrib>Ivanova, Viara</creatorcontrib><creatorcontrib>Tonkova, Alexandra</creatorcontrib><collection>CrossRef</collection><collection>Mechanical &amp; Transportation Engineering Abstracts</collection><collection>Technology Research Database</collection><collection>ANTE: Abstracts in New Technology &amp; Engineering</collection><collection>Engineering Research Database</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><jtitle>Process biochemistry (1991)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Vassileva, Anna</au><au>Beschkov, Venko</au><au>Ivanova, Viara</au><au>Tonkova, Alexandra</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Continuous cyclodextrin glucanotransferase production by free and immobilized cells of Bacillus circulans ATCC 21783 in bioreactors</atitle><jtitle>Process biochemistry (1991)</jtitle><date>2005-10-01</date><risdate>2005</risdate><volume>40</volume><issue>10</issue><spage>3290</spage><epage>3295</epage><pages>3290-3295</pages><issn>1359-5113</issn><eissn>1873-3298</eissn><abstract>Four systems for a continuous cyclodextrin glucanotransferase (CGTase) production by Bacillus circulans ATCC 21783 were studied: free cells in a stirred reactor; free cells in a bubble column reactor; agar-entrapped and membrane-immobilized cells in a bubble column reactor. The performance of the process with free cells led to a higher enzyme level, specific CGTase activity and enzyme productivity compared to systems using immobilized cells. However, the residual enzyme activity (after approximately 300 h process) was from 96 to 97% for immobilized cells and from 78 to 86% for free cells. The four used systems provided a significantly higher CGTase yield (from 3.4-fold to 4.7-fold) and productivity (from 1.4-fold to 2.9-fold) in comparison to known strains of Bacillus applied for the same aim. The concomitant high specific CGTase activity indicated that the established cultural conditions provided the dominant synthesis of the active protein.</abstract><pub>Elsevier Ltd</pub><doi>10.1016/j.procbio.2005.03.022</doi><tpages>6</tpages></addata></record>
fulltext fulltext
identifier ISSN: 1359-5113
ispartof Process biochemistry (1991), 2005-10, Vol.40 (10), p.3290-3295
issn 1359-5113
1873-3298
language eng
recordid cdi_proquest_miscellaneous_29110694
source Elsevier
subjects Bacillus circulans
Bioreactors
Continuous enzyme production
Cyclodextrin glucanotransferase
Cyclodextrins
Free and immobilized cells
title Continuous cyclodextrin glucanotransferase production by free and immobilized cells of Bacillus circulans ATCC 21783 in bioreactors
url http://sfxeu10.hosted.exlibrisgroup.com/loughborough?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-02T12%3A17%3A58IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Continuous%20cyclodextrin%20glucanotransferase%20production%20by%20free%20and%20immobilized%20cells%20of%20Bacillus%20circulans%20ATCC%2021783%20in%20bioreactors&rft.jtitle=Process%20biochemistry%20(1991)&rft.au=Vassileva,%20Anna&rft.date=2005-10-01&rft.volume=40&rft.issue=10&rft.spage=3290&rft.epage=3295&rft.pages=3290-3295&rft.issn=1359-5113&rft.eissn=1873-3298&rft_id=info:doi/10.1016/j.procbio.2005.03.022&rft_dat=%3Cproquest_cross%3E28497207%3C/proquest_cross%3E%3Cgrp_id%3Ecdi_FETCH-LOGICAL-c408t-3d230ebac424e5dcd2320b6a27f9289dab4db017d841a5dafc7ed65bb0af1ed3%3C/grp_id%3E%3Coa%3E%3C/oa%3E%3Curl%3E%3C/url%3E&rft_id=info:oai/&rft_pqid=28497207&rft_id=info:pmid/&rfr_iscdi=true