Changes in calcium absorption and subsequent tissue distribution induced by Maillard reaction products: in vitro and in vivo assays

• Published in: Journal of the science of food and agriculture 2006-01, Vol.86 (2), p.271-278
• Main Authors: Delgado-Andrade, C, Seiquer, I, Navarro, M.P
• Format: Article
• Language: English
• Subjects: animal models; Bioavailability; Biological and medical sciences; blood chemistry; Body weight; bone; Caco-2 cells; Calcium; Cell culture; cell lines; cultured cells; Diet; dietary minerals; digestibility; Digestive system; excretion; feces; feed intake; Food industries; Fundamental and applied biological sciences. Psychology; glucose; heat treatment; Heating; in vitro studies; in vivo studies; lysine; Maillard reaction products; methionine; nutrient availability; nutrient balance; nutrient retention; Nutrition; rats; Retention; Rodents
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.2323

The effects of Maillard reaction products (MRP) from glucose-lysine and glucose-methionine on calcium bioavailability were studied by in vivo (rats) and in vitro (Caco-2 cells) assays. Equimolar glucose/lysine and glucose/methionine mixtures (40% moisture) were heated (150 °C, 30 min) to prepare samples (GL30 and GM30, respectively). For 21 days, rats were fed a control diet (control group) or diets containing separately 3% of the heated mixtures (GL30 and GM30 groups, respectively). In the last week a calcium balance was performed, after which the animals were sacrificed and some organs and serum were removed to analyze calcium levels. A second balance was carried out throughout the experimental period to calculate global calcium retention (retained calcium during the entire 21 days). Unheated and heated samples were used for calcium transport experiments in Caco-2 cells. Food intake and final body weight were lower in the GM30 group. Calcium fecal excretion decreased and digestibility increased in this group. Accordingly, increased calcium transport in Caco-2 cells was found in the presence of the GM30 sample, when compared with the unheated sample. However, global calcium retention tended to decrease in the GM30 group, mainly owing to the lower food intake. Bone calcium concentrations decreased in the animals fed the MRP diets. The possible long-term effects of MRP intake on calcium digestibility and bone calcium should be taken into account to avoid related diseases.