2,3,7,8-TCDD equivalence and mutagenic activity associated with PM10 from three urban locations in New Zealand

• Published in: The Science of the total environment 2005-10, Vol.349 (1-3), p.161-174
• Main Authors: BROWN, Leslie E, TROUGHT, Katherine R, BAILEY, Christopher I, CLEMONS, Janine H
• Format: Article
• Language: English
• Subjects: Air; Air Pollutants - analysis; Air Pollutants - toxicity; Animals; Biological and medical sciences; Cell Line, Tumor; Chemical mutagenesis; Cities; Cytochrome P-450 CYP1A1 - metabolism; Dust - analysis; Environmental pollutants toxicology; Medical sciences; Mutagenicity Tests; New Zealand; Polychlorinated Dibenzodioxins - analysis; Polychlorinated Dibenzodioxins - toxicity; Polycyclic Aromatic Hydrocarbons - analysis; Polycyclic Aromatic Hydrocarbons - toxicity; Rats; Salmonella typhimurium - drug effects; Salmonella typhimurium - genetics; Seasons; Toxicology
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2005.01.008

Ambient particulate matter (PM(10)) in urban centres varies depending on emission sources, geography, demography, and meteorology. Hence physical (PM(10), wind speed, rainfall, temperature), chemical (polycyclic aromatic hydrocarbons, PAH), and toxicological (Ames Test, H4IIE EROD Assay) analyses were done on daily PM(10) (approximately 1640 m(3)/day) collected from three New Zealand urban sites where winter emissions were predominantly due to domestic home heating. Daily PM(10) levels ranged between 9.7 and 20.8 in summer and between 21.8 and 61.0 microg/m(3) in winter. Daily PAH concentrations were 0.5, 0.45, and 1.5 ng/m(3) in summer and 52.1, 128.9, and 5.8 ng/m(3) in winter at sites Christchurch, Alexandra and Dunedin, respectively. During winter, 74% of PM(10) extracts from all three sites showed significant mutagenicity in the Ames Test (TA 98, -S9), whereas approximately 25% of the daily PM(10) was mutagenic in summer. Benzo[a]pyrene and BaP carcinogenic equivalence concentrations during winter were strongly correlated to both mutagenicity and TCDD-like activity at two sites. Daily levels of TCDD toxicity equivalence concentrations ranged from 0.5 to 3.6 pg TCDD/m(3) air in summer and from 0.3 to 4009 pg TCDD/m(3) air in winter. Chemically and biologically derived TCDD toxicity equivalent concentrations were significantly correlated in all study locations indicating that PAH may represent most of the TCDD-like activity present in the PM(10).