Coupling between 2, 2′, 4, 4′-tetrabromodiphenyl ether (BDE-47) debromination and methanogenesis in anaerobic soil microcosms

• Published in: The Science of the total environment 2024-03, Vol.914, p.169831-169831, Article 169831
• Main Authors: Fang, Shasha, Geng, Yue, Wang, Lu, Zeng, Jun, Zhang, Shimin, Wu, Yucheng, Lin, Xiangui
• Format: Article
• Language: English
• Subjects: Dehalogenation; Methane; Methanogens; Organohalide-respiring bacteria; PBDEs
• ISSN: 0048-9697; 1879-1026
• DOI: 10.1016/j.scitotenv.2023.169831

Polybrominated diphenyl ethers (PBDEs) are persistent pollutants that may undergo microbial-mediated debromination in anoxic environments, where diverse anaerobic microbes such as methanogenic archaea co-exist. However, current understanding of the relations between PBDE pollution and methanogenic process is far from complete. To address this knowledge gap, a series of anaerobic soil microcosms were established. BDE-47 (2, 2′, 4, 4′-tetrabromodiphenyl ether) was selected as a model pollutant, and electron donors were supplied to stimulate the activity of anaerobes. Debromination and methane production were monitored during the 12 weeks incubation, while obligate organohalide-respiring bacteria (OHRBs), methanogenic, and the total bacterial communities were examined at week 7 and 12. The results demonstrated slow debromination of BDE-47 in all microcosms, with considerable growth of Dehalococcoides and Dehalogenimonas over the incubation observed in most BDE-47 spiked treatments. In addition, the accumulation of intermediate metabolites positively correlated with the abundance of Dehalogenimonas at week 7, suggesting potential role of these OHRBs in debromination. Methanosarcinaceae were identified as the primary methanogenic archaea, and their abundance were correlated with the production of debrominated metabolites at week 7. Furthermore, it was observed for the first time that BDE-47 considerably enhanced methane production and increased the abundance of mcrA genes, highlighting the potential effects of PBDE pollution on climate change. This might be related to the inhibition of reductive N- and S-transforming microbes, as revealed by the quantitative microbial element cycling (QMEC) analysis. Overall, our findings shed light on the intricate interactions between PBDE and methanogenic processes, and contribute to a better understanding of the environmental fate and ecological implication of PBDE under anaerobic settings. [Display omitted] •Debromination of BDE-47 occurred in anaerobic soil microcosms.•BDE-47 debromination was associated with Dehalogenimonas and Dehalococcoides.•BDE-47 promoted CH4 emission and affected N- and S-transforming microbes.•Methanogenesis was coupled to degradation of BDE-47.