Analysis of phenotypic changes in high temperature and low pH extreme conditions of Alicyclobacillus sendaiensis PA2 related with the cell wall and sporulation genes

• Published in: Archives of microbiology 2024-01, Vol.206 (1), p.53-53, Article 53
• Main Authors: Ortiz-Cortés, Lourdes Yaret, Aréchiga-Carvajal, Elva Teresa, Ventura-Canseco, Lucía María Cristina, Ruíz-Valdiviezo, Victor Manuel, Gutiérrez-Miceli, Federico Antonio, Alvarez-Gutiérrez, Peggy Elizabeth
• Format: Article
• Language: English
• Subjects: Adaptation; Alicyclobacillus; Archives & records; Bacteria; Batch culture; Biochemistry; Biomedical and Life Sciences; Biotechnology; Carbon; Cell Biology; Cell culture; Cell Wall - genetics; Cell walls; Chromosomes; Ecology; Enzymes; Genes; Genomes; Genomic analysis; High temperature; Hydrogen-Ion Concentration; Life Sciences; Lipids; Microbial Ecology; Microbiology; Microorganisms; Nitrogen; Original Paper; Sporulation; Subsystems; Temperature
• ISSN: 0302-8933; 1432-072X
• DOI: 10.1007/s00203-023-03735-1

The A. sendaiensis PA2 is a polyextremophile bacterium. In this study, we analyze the A. sendaiensis PA2 genome. The genome was assembled and annotated. The A. sendaiensis PA2 genome structure consists of a 2,956,928 bp long chromosome and 62.77% of G + C content. 3056 CDSs were predicted, and 2921 genes were assigned to a putative function. The ANIm and ANIb value resulted in 97.17% and 96.65%, the DDH value was 75.5%, and the value of TETRA (Z-score) was 0.98. Comparative genomic analyses indicated that three systems are enriched in A. sendaiensis PA2. This strain has phenotypic changes in cell wall during batch culture at 65 °C, pH 5.0 and without carbon and nitrogen source. The presence of unique genes of cell wall and sporulation subsystem could be related to the adaptation of A. sendaiensis PA2 to hostile conditions.