Industrially produced trans-fatty acids are potent promoters of DNA damage-induced apoptosis

trans-Fatty acids (TFAs) are unsaturated fatty acids harboring at least one carbon-carbon double bond in trans configuration, which are categorized into two groups according to their origin: industrial and ruminant TFAs, hereafter called iTFAs and rTFAs, respectively. Numerous epidemiological studie...

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Bibliographic Details
Published in:Journal of toxicological sciences 2024, Vol.49(1), pp.27-36
Main Authors: Hirata, Yusuke, Kojima, Ryota, Ashida, Ryo, Nada, Yuki, Kimura, Shinnosuke, Sato, Emiko, Noguchi, Takuya, Matsuzawa, Atsushi
Format: Article
Language:English
Subjects:
Apoptosis
Apoptosis - genetics
c-Jun protein
Carbon
Damage
Deoxyribonucleic acid
DNA
DNA Breaks, Double-Stranded
DNA Damage
Doxorubicin
Doxorubicin - toxicity
Epidemiology
Fatty acids
JNK
JNK protein
Kinases
Neurodegenerative diseases
Oleic acid
Reactive oxygen species
Toxicity
Trans fatty acids
Trans Fatty Acids - toxicity
Transcription factors
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Summary:trans-Fatty acids (TFAs) are unsaturated fatty acids harboring at least one carbon-carbon double bond in trans configuration, which are categorized into two groups according to their origin: industrial and ruminant TFAs, hereafter called iTFAs and rTFAs, respectively. Numerous epidemiological studies have shown a specific link of iTFAs to various diseases, such as cardiovascular and neurodegenerative diseases. However, there is little evidence for underlying mechanisms that can explain the specific toxicity of iTFAs, and how to mitigate their toxicity. Herein, we show that iTFAs, including elaidic acid (EA) and linoelaidic acid, but not rTFAs, facilitate apoptosis induced by doxorubicin (Dox), triggering DNA double-strand breaks. We previously established that EA promotes Dox-induced apoptosis by accelerating c-Jun N-terminal kinase (JNK) activation through mitochondrial reactive oxygen species (ROS) overproduction. Consistently, iTFAs specifically enhanced Dox-induced JNK activation. Furthermore, Dox-induced pro-apoptotic signaling by iTFAs was blocked in the presence of oleic acid (OA), the geometrical cis isomer of EA. These results demonstrate that iTFAs specifically exert their toxicity during DNA damage-induced apoptosis, which could be effectively suppressed by OA. Our study provides evidence for understanding the difference in toxic actions between TFA species, and for new strategies to prevent and combat TFA-related diseases.
ISSN:0388-1350
1880-3989
DOI:10.2131/jts.49.27