Evaluation of probiotic bifidobacteria strains from Iranian traditional dairy products for their anti-hyperlipidemic potential

• Published in: Folia microbiologica 2024-08, Vol.69 (4), p.875-887
• Main Authors: Afshar, Nasim, Amini, Kumarss, Mohajerani, Hamidreza, Saki, Sasan
• Format: Article
• Language: English
• Subjects: Animal models; Animals; Anti-Bacterial Agents - pharmacology; Antibacterial activity; Antibiotics; Antiinfectives and antibacterials; Antimicrobial activity; Applied Microbiology; Bifidobacterium - genetics; Bifidobacterium - isolation & purification; Bifidobacterium - metabolism; Bile Acids and Salts - metabolism; Biomedical and Life Sciences; Caco-2 Cells; Cholesterol; Cholesterol - blood; Cholesterol - metabolism; Cholesterol 7-alpha-Hydroxylase - genetics; Cholesterol 7-alpha-Hydroxylase - metabolism; Dairy products; Dairy Products - microbiology; Diet; Disease Models, Animal; Environmental Engineering/Biotechnology; Feces - microbiology; Fermented food; Fermented milk products; High fat diet; Humans; Hyperlipidemias; Immunology; In vivo methods and tests; Iran; Life Sciences; Lipids; Liver; Liver - metabolism; Low density lipoprotein; Male; Microbiology; Original Article; Probiotics; Probiotics - administration & dosage; Probiotics - pharmacology; Rats; Rats, Wistar; RNA, Ribosomal, 16S - genetics; rRNA 16S; Small intestine; Triglycerides; Triglycerides - blood; Triglycerides - metabolism
• ISSN: 0015-5632; 1874-9356; 1874-9356
• DOI: 10.1007/s12223-023-01124-1

This study investigated the therapeutic potential of probiotic bifidobacteria, isolated from Iranian fermented dairy products, in a hyperlipidemic animal model. Bifidobacterium strains were extracted from traditional dairy samples and screened using physiological and phenotypic examinations, 16S rRNA analysis, and probiotic properties such as tolerance to gastrointestinal juice, antimicrobial activity, and antibiotic susceptibility. The ability of the screened bifidobacteria to reduce serum and liver lipids in vivo was tested using male Wistar rats. Six strains of bifidobacteria were isolated from traditional Iranian fermented dairy. These strains showed promising in vitro activity in lowering triglyceride and cholesterol, tolerance to simulated gastrointestinal juice, the ability to adhere to Caco-2 cells, acceptable antibiotic susceptibility, and a broad spectrum of antibacterial activity. The diet supplemented with isolated bifidobacteria significantly reduced serum total cholesterol (TC), triglyceride (TG), low-density lipoprotein cholesterol (LDL-C), liver tissue lipid levels, and hepatic enzymes in animals when compared to a high-fat diet without strains ( p