Qualitative and quantitative rapid detection of VOCs differentially released by VAP-associated bacteria using PTR-MS and FGC-PTR-MS

• Published in: Analyst (London) 2024-02, Vol.149 (5), p.1447-1454
• Main Authors: Xu, Wei, Zou, Xue, Ding, Yueting, Zhang, Qi, Song, Yulan, Zhang, Jin, Yang, Min, Liu, Zhou, Zhou, Qiang, Ge, Dianlong, Zhang, Qiangling, Song, Wencheng, Huang, Chaoqun, Shen, Chengyin, Chu, Yannan
• Format: Article
• Language: English
• Subjects: Acetaldehyde; Bacteria; E coli; Enterobacter cloacae; Gas chromatography; Mass spectrometry; Microorganisms; Pathogens; Principal components analysis; Protons; Scientific imaging; VOCs; Volatile organic compounds
• ISSN: 0003-2654; 1364-5528
• DOI: 10.1039/d3an02011h

Ventilator-associated pneumonia (VAP) is a prevalent disease caused by microbial infection, resulting in significant morbidity and mortality within the intensive care unit (ICU). The rapid and accurate identification of pathogenic bacteria causing VAP can assist clinicians in formulating timely treatment plans. In this study, we attempted to differentiate bacterial species in VAP by utilizing the volatile organic compounds (VOCs) released by pathogens. We cultured 6 common bacteria in VAP in vitro , including Acinetobacter baumannii , Enterobacter cloacae , Escherichia coli , Pseudomonas aeruginosa , Stenotrophomonas maltophilia , and Staphylococcus aureus , which covered most cases of VAP infection in clinic. After the VOCs released by bacteria were collected in sampling bags, they were quantitatively detected by a proton transfer reaction-mass spectrometry (PTR-MS), and the characteristic ions were qualitatively analyzed through a fast gas chromatography-proton transfer reaction-mass spectrometry (FGC-PTR-MS). After conducting principal component analysis (PCA) and analysis of similarities (ANOSIM), we discovered that the VOCs released by 6 bacteria exhibited differentiation following 3 h of quantitative cultivation in vitro . Additionally, we further investigated the variations in the types and concentrations of bacterial VOCs. The results showed that by utilizing the differences in types of VOCs, 6 bacteria could be classified into 5 sets, except for A. baumannii and E. cloacae which were indistinguishable. Furthermore, we observed significant variations in the concentration ratio of acetaldehyde and methyl mercaptan released by A. baumannii and E. cloacae. In conclusion, the VOCs released by bacteria could effectively differentiate the 6 pathogens commonly associated with VAP, which was expected to assist doctors in formulating treatment plans in time and improve the survival rate of patients. Ventilator-associated pneumonia (VAP) is a prevalent disease caused by bacterial infection, and the traditional methods of identifying bacterial strains are time-consuming. In this study, the rapid identification of bacterial strains is expected to be achieved by detecting VOCs released by bacteria.