IiAGL6 participates in the regulation of stamen development and pollen formation in Isatis indigotica

The AGL6 (AGMOUSE LIKE 6) gene is a member of the SEP subfamily and functions as an E-class floral homeotic gene in the development of floral organs. In this study, we cloned IiAGL6, the orthologous gene of AGL6 in Isatis indigotica. The constitutive expression of IiAGL6 in Arabidopsis thaliana resu...

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Published in:Plant science (Limerick) 2024-03, Vol.340, p.111974-111974, Article 111974
Main Authors: Yang, Liu, Qiao, Xin, He, Hao-En, Yi, Wei-Wei, Gao, Ya-Nan, Tan, Xiao-Min, Cheng, Hao, Hou, Xiao-Fang, Ma, Ye-Ye, Wang, Hong-Li, Huang, Xuan, Ma, Yan-Qin, Xu, Zi-Qin
Format: Article
Language:English
Subjects:
AGMOUSE LIKE 6
Arabidopsis thaliana
Development of stamen
Isatis indigotica fortune
Virus-induced gene silencing
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Summary:The AGL6 (AGMOUSE LIKE 6) gene is a member of the SEP subfamily and functions as an E-class floral homeotic gene in the development of floral organs. In this study, we cloned IiAGL6, the orthologous gene of AGL6 in Isatis indigotica. The constitutive expression of IiAGL6 in Arabidopsis thaliana resulted in a late-flowering phenotype and the development of curly leaves during the vegetative growth period. Abnormal changes in floral organ development were observed during the reproductive stage. In woad plants, suppression of IiAGL6 using TRV-VIGS (tobacco rattle virus-mediated virus-induced gene silencing) decreased the number of stamens and led to the formation of aberrant anthers. Similar changes in stamen development were also observed in miRNA-AGL6 transgenic Arabidopsis plants. Yeast two-hybrid and BiFC tests showed that IiAGL6 can interact with other MADS-box proteins in woad; thus, playing a key role in defining the identities of floral organs, particularly during stamen formation. These findings might provide novel insights and help investigate the biological roles of MADS transcription factors in I. indigotica. •IiAGL6 was the orthologous gene of AGL6 in Isatis indigotica.•IiAGL6 transgenic Arabidopsis plants were late-flowering and produce curly leaves.•IiAGL6 overexpression resulted in abnormal changes in floral organs.•Suppression of IiAGL6 using TRV-VIGS led to aberrant stamens.•IiAGL6 play a crucial role in defining the identities of stamens.
ISSN:0168-9452
1873-2259
DOI:10.1016/j.plantsci.2024.111974