Structural modifications of INGAP-PP present in HTD4010 peptide potentiate its effect on rat islet gene expression and insulin secretion

• Published in: Peptides (New York, N.Y. : 1980) N.Y. : 1980), 2024-03, Vol.173, p.171148-171148, Article 171148
• Main Authors: Algañarás, Macarena, Román, Carolina L., Gagliardino, Juan J., Maiztegui, Bárbara, Flores, Luis E.
• Format: Article
• Language: English
• Subjects: Diabetes; Gene expression; INGAP-PP; Insulin secretion; Prediabetes
• ISSN: 0196-9781; 1873-5169
• DOI: 10.1016/j.peptides.2024.171148

Type 2 diabetes (T2D) is characterized by peripheral insulin resistance and altered insulin secretion due to a progressive loss of β-cell mass and function. Today, most antidiabetic agents are designed to resolve impaired insulin secretion and/or insulin resistance, and only GLP-1-based formulations contribute to stopping the decline in β-cell mass. HTD4010, a peptide carrying two modifications of the amino acid sequence of INGAP-PP (N-terminus acetylation and substitution of Asn13 by Ala) showed greater plasma stability and could be a good candidate for proposal as a drug that could improve β cell mass and function lost in T2D. In the present study, we showed that HTD4010 included in the culture media of normal rat islets at a dose 100 times lower than that used for INGAP-PP was able to modulate, in the same way as the original peptide, both insulin secretion in response to glucose and the expression of key genes related to insular function, insulin and leptin intracellular pathways, neogenesis, apoptosis, and inflammatory response. Our results confirm the positive effect of HTD4010 on β-cell function and gene expression of factors involved in the maintenance of β-cell mass. Although new assays in animal models of prediabetes and T2D must be performed to be conclusive, our results are very encouraging, and they suggest that the use of HTD4010 at a dose 100 times lower than that of INGAP-PP could minimize its side effects in a future clinical trial. •In vitro effects of HTD4010 on rat islets are similar to those from INGAP-PP.•HTD4010 increases glucose-stimulated insulin secretion in a dose-response manner.•HTD4010 potentiates gene expression of key factors that modulate β-cell mass.•In addition to being more stable, HTD4010 is 100 times more potent than INGAP-PP.•It could be tested as an antidiabetic agent with a low risk of secondary effects.