Comparison of PROSPECT and HPLC estimates of leaf chlorophyll contents in a grapevine stress study

This letter describes how PROSPECT, updated with a 1 nm chlorophyll absorbance database, was used to infer total chlorophyll content from the measured reflectance spectra of grapevine leaves. Modelled and measured reflectance spectra agreed on average to within 13% over the range 400-1600 nm. Howeve...

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Bibliographic Details
Published in:International journal of remote sensing 2006-02, Vol.27 (4), p.817-823
Main Authors: Renzullo, L. J., Blanchfield, A. L., Guillermin, R., Powell, K. S., Held, A. A.
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
Applied geophysics
Biological and medical sciences
Earth sciences
Earth, ocean, space
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Generalities. Biometrics, experimentation. Remote sensing
Internal geophysics
Remote sensing
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Summary:This letter describes how PROSPECT, updated with a 1 nm chlorophyll absorbance database, was used to infer total chlorophyll content from the measured reflectance spectra of grapevine leaves. Modelled and measured reflectance spectra agreed on average to within 13% over the range 400-1600 nm. However, discrepancies between modelled and measured reflectances of up to 40% were observed in the spectral region dominated by chlorophyll content. In terms of estimating total chlorophyll content, PROSPECT gave estimates that were on average within 10 µg cm −2 of those obtained using high performance liquid chromatography.
ISSN:0143-1161
1366-5901
DOI:10.1080/01431160500239164