A sensitive coumarin fluorescence sensor designed for isoprene detection and imaging research in plants

The release of isoprene by plants is considered to be an adaptation to the environment. Herein, a highly selective coumarin fluorescent probe (DMIC) was designed for detecting isoprene. When isoprene came into contact with the maleimide of DMIC, an electrophilic addition process took place. The powe...

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Bibliographic Details
Published in:Biosensors & bioelectronics 2024-03, Vol.248, p.115998-115998, Article 115998
Main Authors: Liu, Zhixin, Wang, Mengyuan, Wu, Meng, Yu, Xueling, Sun, Qijun, Su, Chenglin, Sun, Yining, Cao, Shuang, Niu, Na, Chen, Ligang
Format: Article
Language:English
Subjects:
Biosensing Techniques
Butadienes
Coumarins
Hemiterpenes
Plants
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Summary:The release of isoprene by plants is considered to be an adaptation to the environment. Herein, a highly selective coumarin fluorescent probe (DMIC) was designed for detecting isoprene. When isoprene came into contact with the maleimide of DMIC, an electrophilic addition process took place. The powerful push-pull effect of DMIC was disrupted. Simultaneously, intramolecular charge transfer was initiated. This enabled DMIC to achieve rapid detection of isoprene within 5 min. Furthermore, excellent linearity was observed in the concentration range of 1-560 ppm (R  = 0.996). A limit of detection is 1.6 ppm. DMIC was applied to in vitro studies of plant release of liberated isoprene. By monitoring the release of isoprene from different tree species throughout the day, the dynamics of isoprene release from plants throughout the day have been successfully revealed. In addition, the release of isoprene varied considerably among different tree species. In particular, the biocompatibility of DMIC allowed for the in vivo detection of isoprene using fluorescence imaging. The results successfully revealed the dynamics of isoprene release in plants under stress. The amount of isoprene that a plant produced increased with the severity of the stress it experienced. This suggested that the level of isoprene content in plants could be used as a preliminary indicator of the physiological health status of plants. This research demonstrates great potential for clarifying signal transduction in biological systems. It provided ideas for further understanding the biology of isoprene.
ISSN:0956-5663
1873-4235
DOI:10.1016/j.bios.2024.115998