Magnetic enzyme reactors for isolation and study of heterogeneous glycoproteins

The newly developed magnetic micro- and nanoparticles with defined hydrophobicity and porosity were used for the preparation of magnetic enzyme reactors. Magnetic particles with immobilized proteolytic enzymes trypsin, chymotrypsin and papain and with enzyme neuraminidase were used to study the stru...

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Bibliographic Details
Published in:Journal of magnetism and magnetic materials 2005-05, Vol.293 (1), p.349-357
Main Authors: Korecká, Lucie, Ježová, Jana, Bílková, Zuzana, Beneš, Milan, Horák, Daniel, Hradcová, Olga, Slováková, Marcela, Viovy, Jean-Louis
Format: Article
Language:English
Subjects:
Chymotrypsin
Condensed matter: electronic structure, electrical, magnetic, and optical properties
Enzymes
Exact sciences and technology
Glycoproteins
Magnetic enzyme reactor (IMER)
Magnetic properties and materials
Magnetic separation
Neuraminidase
Papain
Physics
Small particles and nanoscale materials
Studies of specific magnetic materials
Trypsin
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Summary:The newly developed magnetic micro- and nanoparticles with defined hydrophobicity and porosity were used for the preparation of magnetic enzyme reactors. Magnetic particles with immobilized proteolytic enzymes trypsin, chymotrypsin and papain and with enzyme neuraminidase were used to study the structure of heterogeneous glycoproteins. Factors such as the type of carrier, immobilization procedure, operational and storage stability, and experimental conditions were optimized.
ISSN:0304-8853
DOI:10.1016/j.jmmm.2005.02.030