Assessment of the ecological quality status of the Sepetiba Bay (SE Brazil): When metabarcoding meets morphology on foraminifera

• Published in: Marine environmental research 2024-03, Vol.195, p.106340-106340, Article 106340
• Main Authors: Damasceno, Fabrício Leandro, Alves Martins, Maria Virgínia, Frontalini, Fabrizio, Pawlowski, Jan, Cermakova, Kristina, Angeles, Inès Barrenechea, Costa Santos, Luiz Guilherme, Filho, João Graciano Mendonça, Francescangeli, Fabio, Senez-Mello, Thaise M., Castelo, Wellen Fernanda Louzada, Rebouças, Renata Cardia, Duleba, Wania, Mello e Sousa, Silvia Helena de, Laut, Lazaro, Antonioli, Luzia, Carvalho Da Silva, André Luiz, Ramos e Silva, Carlos Augusto, Vilela, Claudia Gutterres, Dias, Fabio Ferreira, Coe, Heloísa Helena Gomes, Castro, João Wagner Alencar, Crapez, Mirian Araújo Carlos, Damasceno, Raimundo, Dino, Rodolfo, Carelli, Thiago Gonçalves, Reis, Antonio Tadeu dos, Guerra, Josefa Varela
• Format: Article
• Language: English
• Subjects: Diversity; eDNA; Foram-AMBI; FSI; Metals; Organic matter enrichment; Pollution
• ISSN: 0141-1136; 1879-0291
• DOI: 10.1016/j.marenvres.2024.106340

In recent years, the region surrounding Sepetiba Bay (SB; SE Brazil) has become a hub of intense urban expansion and economic exploitation in response to ore transport and industrial and port activities. As a result, contaminants have been introduced into the bay, leading to an overall worsening of the environmental quality. The present work applies for the first time a foraminiferal morphology-based approach (M) and eDNA-based metabarcoding sequencing (G), along with geochemical data to assess the ecological quality status (EcoQS) in the SB. Principal component analysis shows that the eDNA and morphospecies diversity as well as most of the taxa relative abundance decline in response to the environmental stress (ES) gradient related to total organic carbon (TOC) and metal pollution. Based on ecological indices, Exp(H'bc) (G), Exp(H'bc) (M), foraminifera ATZI marine biotic index (Foram-AMBI), Foram Stress Index (FSI), and geochemical indices (TOC and Potential Ecological Risk Index), the lowest values of EcoQS (i.e., bad to moderate) are inferred in the innermost part of the SB. Despite minor discrepancies among the six EcoQS indices, an agreement has been found for 63% of the stations. To improve the agreement between the ecological indices, it is necessary to fill the gap in species ecology; information on the ecology of many species is still unknown. This work reinforces the importance of molecular analysis and morphological methods in environmental impact studies and confirms the reliability of foraminiferal metabarcoding in EcoQS assessment. This is the first study evaluating the EcoQS in the South Atlantic by using combined foraminiferal eDNA metabarcoding with morphological data. •Foraminiferal eDNA and morphology are used to infer EcoQS in coastal SE Brazil.•Biotic and abiotic indices show poor/bad EcoQS in the inner area of the Sepetiba Bay.•High/good EcoQS are found in the outer area of the Sepetiba Bay.•Molecular and morpho-methods provide similar EcoQS.