Seeking arrangements: cell contact as a cleavage-stage biomarker

• Published in: Reproductive biomedicine online 2024-03, Vol.48 (3), p.103654, Article 103654
• Main Authors: He, Chloe, Karpavičiūtė, Neringa, Hariharan, Rishabh, Lees, Lilly, Jacques, Céline, Ferrand, Timothy, Chambost, Jérôme, Wouters, Koen, Malmsten, Jonas, Miller, Ryan, Zaninovic, Nikica, Vasconcelos, Francisco, Hickman, Cristina
• Format: Article
• Language: English
• Subjects: 3D reconstruction; Abortion, Spontaneous; Aneuploidy; Artificial Intelligence; Biomarkers; Blastocyst; Cell arrangement; Cell contact; Cleavage stage; Cleavage Stage, Ovum; Embryo Transfer - methods; Embryology; Female; Humans; Network analysis; Pilot Projects; Pregnancy; Pregnancy Rate; Retrospective Studies
• ISSN: 1472-6483; 1472-6491; 1472-6491
• DOI: 10.1016/j.rbmo.2023.103654

[Display omitted] •Increased cell contact is associated with greater developmental potential in embryos.•This association is strongest at the eight-cell stage.•Three-dimensional embryo analysis may provide new insights into embryo development. What can three-dimensional cell contact networks tell us about the developmental potential of cleavage-stage human embryos? This pilot study was a retrospective analysis of two Embryoscope imaging datasets from two clinics. An artificial intelligence system was used to reconstruct the three-dimensional structure of embryos from 11-plane focal stacks. Networks of cell contacts were extracted from the resulting embryo three-dimensional models and each embryo's mean contacts per cell was computed. Unpaired t-tests and receiver operating characteristic curve analysis were used to statistically analyse mean cell contact outcomes. Cell contact networks from different embryos were compared with identical embryos with similar cell arrangements. At t4, a higher mean number of contacts per cell was associated with greater rates of blastulation and blastocyst quality. No associations were found with biochemical pregnancy, live birth, miscarriage or ploidy. At t8, a higher mean number of contacts was associated with increased blastocyst quality, biochemical pregnancy and live birth. No associations were found with miscarriage or aneuploidy. Mean contacts at t4 weakly correlated with those at t8. Four-cell embryos fell into nine distinct cell arrangements; the five most common accounted for 97% of embryos. Eight-cell embryos, however, displayed a greater degree of variation with 59 distinct cell arrangements. Evidence is provided for the clinical relevance of cleavage-stage cell arrangement in the human preimplantation embryo beyond the four-cell stage, which may improve selection techniques for day-3 transfers. This pilot study provides a strong case for further investigation into spatial biomarkers and three-dimensional morphokinetics.