Effects of aligned PLGA/SrCSH composite scaffolds on in vitro growth and osteogenic differentiation of human mesenchymal stem cells

Strontium (Sr) has important functions in bone remodeling. Incorporating strontium‐doped α‐calcium sulfate hemihydrate (SrCSH) into poly(lactic‐co‐glycolic acid) (PLGA) fibrous scaffolds were expected to increase its bio‐activity and provide a potential material for bone tissue engineering. In the p...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part B, Applied biomaterials Applied biomaterials, 2024-01, Vol.112 (1), p.e35366-n/a
Main Authors: Liu, Lichu, Luo, Pin, Liao, Honghong, Yang, Kuangyang, Yang, Shenyu, Tu, Mei
Format: Article
Language:English
Subjects:
aligned fibrous scaffold
Biological activity
Biomedical materials
Bone remodeling
Calcium sulfate
Calcium sulfate hemihydrate
Cell adhesion
Cell differentiation
Cholecystokinin
Differentiation (biology)
Fibers
Glycolic acid
Materials research
Materials science
Mesenchymal stem cells
Mineralization
osteogenic differentiation
PLGA
Polylactide-co-glycolide
Scaffolds
Stem cells
Strontium
Tissue engineering
Umbilical cord
Wet spinning
α‐calcium sulfate hemihydrate
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Summary:Strontium (Sr) has important functions in bone remodeling. Incorporating strontium‐doped α‐calcium sulfate hemihydrate (SrCSH) into poly(lactic‐co‐glycolic acid) (PLGA) fibrous scaffolds were expected to increase its bio‐activity and provide a potential material for bone tissue engineering. In the present study, Sr‐containing aligned PLGA/SrCSH fibrous scaffolds similar to the architecture of natural bone were prepared via wet spinning. CCK‐8 assay revealed that Sr‐containing scaffolds possessed better bioactivity and supported favorable cell growth effectively. The aligned PLGA/SrCSH fibers exerted a contact effect on cell attachment, inducing regular cell alignment and influencing a series of cell behaviors. Releasing of high concentration Sr from a‐PLGA/SrCSH scaffolds could induce high expression levels of BMP‐2, increase ALP activity and upregulate RUNX‐2 expression, and further promote the expressions of COL‐I and OCN and the maximum mineralization. This study demonstrated that Sr and ordered structure in a‐PLGA/SrCSH fibrous scaffolds could synergistically enhance the osteogenic differentiation of umbilical cord mesenchymal stem cells (UCMSCs) by regulating cell arrangement and expressions of osteogenic genes.
ISSN:1552-4973
1552-4981
DOI:10.1002/jbm.b.35366