Quantifying adsorption of heparin on a PVC substrate using ATR-FTIR

• Published in: Polymer international 2005-01, Vol.54 (1), p.209-214
• Main Authors: Harada, Nikolas S, Oyama, Helena T, Bártoli, Julio R, Gouvêa, Douglas, Cestari, Idágene A, Hui Wang, S
• Format: Article
• Language: English
• Subjects: Applied sciences; Biological and medical sciences; Exact sciences and technology; Forms of application and semi-finished materials; heparin; infrared spectroscopy; Medical sciences; poly(vinyl chloride); Polymer industry, paints, wood; PVC; Sheets and films; surface modification; Surgery (general aspects). Transplantations, organ and tissue grafts. Graft diseases; Technology of polymers; Technology. Biomaterials. Equipments
• ISSN: 0959-8103; 1097-0126
• DOI: 10.1002/pi.1685

The surface modification of poly(vinyl chloride) (PVC) tubing by heparin was performed to increase its blood compatibility. A solution of benzalkonium heparinate was used in the treatment of two types of plasticized PVC. The modification of the PVC surface was monitored after various treatment conditions, and quantitative results were obtained by using ATR‐FTIR spectroscopy. The treatment times and the PVC type have a strong influence on the observed amount of heparin. A partial removal (20–30 %) of heparin was observed after rinsing the PVC surface with a 0.9 wt% NaCl aqueous solution. The more flexible PVC tubing, having a lower Tg, had a higher concentration of heparin. Final heparin concentrations on the PVC surfaces were found to be in the range 1–17 µg cm−2. Copyright © 2004 Society of Chemical Industry