Photo- and X-ray Induced Cytotoxicity of CeF3-YF3-TbF3 Nanoparticle-Polyvinylpyrrolidone—“Radachlorin” Composites for Combined Photodynamic Therapy

• Published in: Materials 2024-01, Vol.17 (2), p.316
• Main Authors: Khusainova, Alina I., Nizamutdinov, Alexey S., Shamsutdinov, Nail I., Kalinichenko, Svetlana, Safin, Damir I., Gafurov, Marat, Lukinova, Elena V., Batygov, Sergey Kh, Kuznetsov, Sergey V., Zinchenko, Sergey V., Zelenikhin, Pavel V., Pudovkin, Maksim
• Format: Article
• Language: English
• Subjects: Cytotoxicity; Electron microscopes; Energy; Energy transfer; Fluorides; Lasers; Nanocomposites; Nanoparticles; Photodynamic therapy; Polyvinylpyrrolidone; Radiation; Toxicity; X ray irradiation
• ISSN: 1996-1944; 1996-1944
• DOI: 10.3390/ma17020316

The Ce0.5Y0.35Tb0.15F3 nanoparticles with a CeF3 hexagonal structure were synthesized using the co-precipitation technique. The average nanoparticle diameter was 14 ± 1 nm. The luminescence decay curves of the Ce0.5Y0.35Tb0.15F3 nanoparticles (λem = 541 nm, 5D4–7F5 transition of Tb3+) conjugated with Radachlorin using polyvinylpyrrolidone coating as well as without Radachlorin were detected. Efficient nonradiative energy transfer from Tb3+ to the Radachlorin was demonstrated. The maximum energy transfer coefficients for the nanoparticles conjugated with Radachlorin via polyvinylpyrrolidone and without the coating were 82% and 55%, respectively. The average distance between the nanoparticle surface and Radachlorin was R0 = 4.5 nm. The best results for X-ray-induced cytotoxicity were observed for the NP-PVP-Rch sample at the lowest Rch concentration. In particular, after X-ray irradiation, the survival of A549 human lung carcinoma cells decreased by ~12%.