Osteogenic Effect of Genistein on In Vitro Bone Formation by Human Bone Marrow Cell Culture - For Development of Advanced Bio-Artificial Bone

The effect of genistein, a soybean isoflavone, on new bone formation by bone marrow cells from mature humans was examined. After informed consent was obtained from a 55-year-old woman with osteoporosis and lumbar spondylosis deformans，bone marrow cells were collected from her ilium, cultured in the...

Full description

Saved in:
Bibliographic Details
Published in:Key engineering materials 2005-04, Vol.284-286, p.667-670
Main Authors: Takakura, Yoshinori, Iida, Jin, Okumura, Noriko, Nonomura, Akitaka, Yoshikawa, Takafumi
Format: Article
Language:English
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The effect of genistein, a soybean isoflavone, on new bone formation by bone marrow cells from mature humans was examined. After informed consent was obtained from a 55-year-old woman with osteoporosis and lumbar spondylosis deformans，bone marrow cells were collected from her ilium, cultured in the standard medium of MEM containing fetal calf serum and then cultured with or without the addition of genistein to the bone-forming medium containing dexamethasone etc.． In humans, when genistein was added to the bone-forming medium, genistein (10-7 M and 10-8 M)caused a significant increase in the levels of alkaline phosphatase avtivity and DNA content compared with cells not cultured in genistein． In conclusion，genistein was found to promote bone formation at lower concentrations，and thus may be useful as a bone formation-promoting factor.
ISSN:1013-9826
1662-9795
1662-9795
DOI:10.4028/www.scientific.net/KEM.284-286.667