Tannic acid alleviates ETEC K88‐induced intestinal damage through regulating the p62‐keap1‐Nrf2 and TLR4‐NF‐κB‐NLRP3 pathway in IPEC‐J2 cells

• Published in: Journal of the science of food and agriculture 2024-07, Vol.104 (9), p.5186-5196
• Main Authors: Liu, Wenhui, Guo, Kangkang
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants; Barriers; Biocompatibility; Cell Line; Cytokines; Cytotoxicity; Damage prevention; Diamines; Diarrhea; Diarrhea - drug therapy; Diarrhea - microbiology; Dietary supplements; E coli; Enterotoxigenic Escherichia coli; Enzymatic activity; Enzyme activity; Epithelial cells; Epithelial Cells - drug effects; Epithelial Cells - metabolism; Epithelium; Escherichia coli Infections - drug therapy; Escherichia coli Infections - microbiology; ETEC K88; Immunoglobulins; Inflammation; Inflammatory response; Intestinal Mucosa - drug effects; Intestinal Mucosa - metabolism; Intestinal Mucosa - microbiology; Intestine; Intestines - drug effects; Intestines - microbiology; Kelch-Like ECH-Associated Protein 1 - genetics; Kelch-Like ECH-Associated Protein 1 - metabolism; Mechanical properties; mRNA; MyD88 protein; NF-E2-Related Factor 2 - genetics; NF-E2-Related Factor 2 - metabolism; NF-kappa B - genetics; NF-kappa B - metabolism; NLR Family, Pyrin Domain-Containing 3 Protein - metabolism; Oxidative stress; p62‐Nrf2‐keap1; Polyphenols; post‐weaning diarrhea; Signal transduction; Signal Transduction - drug effects; Swine; Swine Diseases - drug therapy; Swine Diseases - metabolism; Swine Diseases - microbiology; Tannic acid; Tannins - pharmacology; TLR4 protein; TLR4‐NF‐κB‐NLRP3; Toll-Like Receptor 4 - genetics; Toll-Like Receptor 4 - metabolism; Toll-like receptors; Weaning
• ISSN: 0022-5142; 1097-0010
• DOI: 10.1002/jsfa.13343

BACKGROUND Tannic acid (TA), a naturally occurring polyphenol, has shown diverse potential in preventing intestinal damage in piglet diarrhea induced by Enterotoxigenic Escherichia coli (ETEC) K88. However, the protective effect of TA on ETEC k88 infection‐induced post‐weaning diarrhea and its potential mechanism has not been well elucidated. Therefore, an animal trial was carried out to investigate the effects of dietary supplementation with TA on the intestinal diarrhea of weaned piglets challenged with ETEC K88. In addition, porcine intestinal epithelial cells were used as an in vitro model to explore the mechanism through which TA alleviates intestinal oxidative damage and inflammation. RESULTS The results indicated that TA supplementation (2 and 4 g kg−1) reduced diarrhea rate, enzyme activity (diamine oxidase [DAO] and Malondialdehyde [MAD]) and serum inflammatory cytokines concentration (TNF‐α and IL‐1β) (P