Klebsiella aerogenes ingestion elicits behavioral changes and innate immunity in the host, Caenorhabditis elegans

• Published in: Developmental and comparative immunology 2024-05, Vol.154, p.105138-105138, Article 105138
• Main Authors: Gowripriya, Thirumugam, Yashwanth, Radhakrishnan, James Prabhanand, Bhaskar, Suresh, Ramamurthi, Balamurugan, Krishnaswamy
• Format: Article
• Language: English
• Subjects: C. elegans; Host-bacterial interaction; K. aerogenes; Western blot
• ISSN: 0145-305X; 1879-0089
• DOI: 10.1016/j.dci.2024.105138

Klebsiella aerogenes (previously known as Enterobacter aerogenes) is a common opportunistic pathogen that infect the respiratory tract and central nervous system. However, how it interferes the host regulatory mechanism has not been previously described. When C. elegans were exposed to K. aerogenes, they exhibited a shorter lifespan compared to those fed with E. coli OP50. The time required for 50 % of L4 hermaphrodite nematodes to die when exposed to K. aerogenes was approximately 9 days, whereas it was about 18 days when fed with E. coli OP50. The interaction with K. aerogenes also affected the physical activity of C. elegans. Parameters like pharyngeal pumping, head thrashing, body bending, and swimming showed a gradual decline during infection. The expression of serotonin-mediated axon regeneration K. aerogenes infection led to increased levels of reactive oxygen species (ROS) in C. elegans compared to E. coli OP50-fed worms. The nematodes activated antioxidant mechanisms, including the expression of SODs, to counteract elevated ROS levels. The interaction with K. aerogenes activated immune regulatory pathways in C. elegans, including the mTOR signaling pathway downstream player SGK-1. Lifespan regulatory pathways, such as pha-4 and pmk-1, were also affected, likely contributing to the nematode ability to survive in a pathogenic environment. K. aerogenes infection has a detrimental impact on the healthspan and lifespan of C. elegans, affecting physical activity, intestinal health, serotonin regulation, ROS levels, and immune responses. These findings provide insights into the complex interactions between K. aerogenes and host organisms. •Klebsiella aerogenes leads to a significant increase in mortality.•The host chemotactic behaviour was affected through serotonin regulation.•K. aerogenes severely affects the health span of host.•C. elegans activates expression of MAPK pathway players such as pmk-1 and SGK-1.