DNA amplification on a PDMS–glass hybrid microchip

• Published in: Journal of micromechanics and microengineering 2006-02, Vol.16 (2), p.425-433
• Main Authors: Niu, Zhi Qiang, Chen, Wen Yuan, Shao, Shi Yi, Jia, Xiao Yu, Zhang, Wei Ping
• Format: Article
• Language: English
• Subjects: Applied fluid mechanics; Applied sciences; Biological and medical sciences; Biotechnology; Exact sciences and technology; Fluid dynamics; Fluidics; Fundamental and applied biological sciences. Psychology; Fundamental areas of phenomenology (including applications); Genetic engineering; Genetic technics; In vitro gene amplification. Pcr technique; Instruments, apparatus, components and techniques common to several branches of physics and astronomy; Mechanical engineering. Machine design; Mechanical instruments, equipment and techniques; Methods. Procedures. Technologies; Micromechanical devices and systems; Physics; Precision engineering, watch making
• ISSN: 0960-1317; 1361-6439
• DOI: 10.1088/0960-1317/16/2/029

In this paper, the development of a hybrid polydimethylsiloxane (PDMS)-glass microchip for PCR (polymerase chain reaction) was reported. A novel temperature control strategy was applied for precise temperature control. The microchip was composed of two parts-a PDMS substrate and a glass cover. The PDMS substrate includes a reaction chamber for PCR and two microchannels for the inlet and the outlet of the sample. A glass cover integrating heaters and sensors was bonded to the PDMS substrate. The microchip was produced by molding PDMS against a microfabricated master with comparatively simple and inexpensive methods. PCR was successfully carried out on the PDMS-glass hybrid microchip with a 183 bp target of DNA.