Tmem119 is involved in bone anabolic effects of PTH through enhanced osteoblastic bone formation in mice

• Published in: Bone (New York, N.Y.) N.Y.), 2024-04, Vol.181, p.117040-117040, Article 117040
• Main Authors: Kawao, Naoyuki, Matsumura, Daichi, Yamada, Ayaka, Okumoto, Katsumi, Ohira, Takashi, Mizukami, Yuya, Hashimoto, Daiki, Kaji, Hiroshi
• Format: Article
• Language: English
• Subjects: Bone formation; Mineralization; PTH; Tmem119
• ISSN: 8756-3282; 1873-2763
• DOI: 10.1016/j.bone.2024.117040

The intermittent administration of parathyroid hormone (PTH) exerts potent bone anabolic effects, which increase bone mineral density (BMD) and reduce fracture risk in osteoporotic patients. However, the underlying mechanisms remain unclear. Tmem119 has been proposed as a factor that is closely linked to the osteoblast phenotype, and we previously reported that PTH enhanced the expression of Tmem119 in mouse osteoblastic cells. However, roles of Tmem119 in the bone anabolic effects of PTH in vivo remain unknown. We herein investigated the roles of Tmem119 in bone anabolic effects of PTH using Tmem119-deficient mice. Tmem119 deficiency significantly reduced PTH-induced increases in trabecular bone volume and cortical BMD of femurs. Effects of Tmem119 deficiency on bone mass seemed predominant in female mice. Histomorphometric analyses with calcein labeling showed that Tmem119 deficiency significantly attenuated PTH-induced increases in the rates of bone formation and mineralization as well as numbers of osteoblasts. Moreover, Tmem119 deficiency significantly blunted PTH-induced decreases in phosphorylation of β-catenin and increases in alkaline phosphatase activity in osteoblasts. In conclusion, the present results indicate that Tmem119 is involved in bone anabolic effects of PTH through osteoblastic bone formation partly related to canonical Wnt-β-catenin signaling in mice. •Tmem119 deficiency reduced trabecular bone volume and cortical BMD enhanced by PTH in mice.•Tmem119 deficiency attenuated bone formation and mineralization enhanced by PTH in mice.•Tmem119 deficiency suppressed PTH-enhanced Wnt/β-catenin signaling and ALP activity in primary osteoblasts.•The results suggest that Tmem119 is involved in bone anabolic effects of PTH in mice.