Algerian Propolis from Distinct Geographical Locations: Chemical Profiles, Antioxidant Capacity, Cytotoxicity and Inhibition of 5‐Lipoxygenase Product Biosynthesis

Propolis was collected from honeybee hives in three geographically distinct Algerian climates and extracts were characterized for composition and bioactivity. Bees were identified as native subspecies using an in‐silico DraI mtDNA COI‐COII test. Over 20 compounds were identified in extracts by LC‐MS...

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Published in:Chemistry & biodiversity 2024-04, Vol.21 (4), p.e202301758-n/a
Main Authors: Ayad, Ahmed Sabri, Hébert, Mathieu P. A., Doiron, Jérémie A., Loucif‐Ayad, Wahida, Daas, Tarek, Smagghe, Guy, Alburaki, Mohamed, Barnett, David A., Touaibia, Mohamed, Surette, Marc E.
Format: Article
Language:English
Subjects:
Algerian honey bees
Anti-inflammatory
Antioxidants
Apoptosis
Biocompatibility
Biological activity
Biosynthesis
Cytotoxicity
Flavonoids
Geographical locations
In vivo methods and tests
Leukemia
Linoleic acid
Lipoxygenase
Mitochondrial DNA
Oxidation
Phenolic compounds
Phenols
Propolis
Scavenging
Tumor cell lines
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Summary:Propolis was collected from honeybee hives in three geographically distinct Algerian climates and extracts were characterized for composition and bioactivity. Bees were identified as native subspecies using an in‐silico DraI mtDNA COI‐COII test. Over 20 compounds were identified in extracts by LC‐MS. Extracts from the Medea region were more enriched in phenolic content (302±28 mg GAE/g of dry extract) than those from Annaba and Ghardaia regions. Annaba extracts had the highest flavonoid content (1870±385 mg QCE/g of dry extract). Medea extracts presented the highest free‐radical scavenging activity (IC50=13.5 μg/mL) using the DPPH radical assay while Ghardaia extracts from the desert region were weak (IC50>100 μg/mL). Antioxidant activities measured using AAPH oxidation of linoleic acid were similar in all extracts with IC50 values ranging from 2.9 to 4.9 μg/mL. All extracts were cytotoxic (MTT assay) and proapoptotic (Annexin‐V) against human leukemia cell lines in the low μg/mL range, although the Annaba extract was less active against the Reh cell line. Extracts inhibited cellular 5‐lipoxygenase product biosynthesis with IC50 values ranging from 0.6 to 3.2 μg/mL. Overall, examined propolis extracts exhibited significant biological activity that warrant further characterization in cellular and in vivo models.
ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.202301758