Inhibitory effects of bioactive compounds on UVB-induced photodamage in human keratinocytes: modulation of MMP1 and Wnt signaling pathways

• Published in: Photochemical & photobiological sciences 2024-03, Vol.23 (3), p.463-478
• Main Authors: Liu, Meiling, Huang, Shaokai, Park, Sunmin
• Format: Article
• Language: English
• Subjects: beta Catenin - metabolism; beta Catenin - pharmacology; Biochemistry; Biomaterials; Cell Line; Chemistry; Chemistry and Materials Science; Collagen - pharmacology; Humans; Keratinocytes - metabolism; Matrix Metalloproteinase 1 - metabolism; Matrix Metalloproteinase 1 - pharmacology; Original Papers; Physical Chemistry; Plant Sciences; Reactive Oxygen Species - metabolism; Skin Aging; Ultraviolet Rays; Wnt Signaling Pathway
• ISSN: 1474-905X; 1474-9092
• DOI: 10.1007/s43630-023-00531-0

UVB radiation significantly threatens skin health, contributing to wrinkle formation and an elevated risk of skin cancer. This study aimed to explore bioactive compounds with potential UVB-protective properties. Using in silico analysis, we chose compounds to reduce binding energy with matrix metalloproteinase-1 (MMP1). Piperitoside, procyanidin C1, and mulberrofuran E emerged as promising candidates through this computational screening process. We investigated the UVB-protective efficacy of the selected compounds and underlying mechanisms in human immortalized keratinocytes (HaCaT). We also investigated the molecular pathways implicated in their action, focusing on the transforming growth factor (TGF)-β and wingless-related integration site (Wnt)/β-catenin signaling pathways. In UVB-exposed HaCaT cells (100 mJ/cm 2 for 30 min), piperitoside, procyanidin C1, and mulberrofuran E significantly reduced reactive oxygen species (ROS) and lipid peroxides, coupled with an augmentation of collagen expression. These compounds suppressed MMP1, tumor necrosis factor-alpha ( TNF-α ), and inducible nitric oxide synthase ( iNOS ) expression, while they concurrently enhanced collagen-1 ( COL1A1 ), β-catenin ( CTNNB1 ), and superoxide dismutase type-1 ( SOD1 ) expression. Furthermore, Wnt/β-catenin inhibitors, when administered subsequently, partially counteracted the reduction in MMP1 expression and alleviated inflammatory and oxidative stress markers induced by the bioactive compounds. In conclusion, piperitoside, procyanidin C1, and mulberrofuran E protected against UVB-induced damage in HaCaT cells by inhibiting MMP1 expression and elevating β-catenin expression. Consequently, these bioactive compounds emerge as promising preventive agents for UVB-induced skin damage, promoting skin health.