A distinct cortical code for socially learned threat

• Published in: Nature (London) 2024-02, Vol.626 (8001), p.1066-1072
• Main Authors: Silverstein, Shana E., O’Sullivan, Ruairi, Bukalo, Olena, Pati, Dipanwita, Schaffer, Julia A., Limoges, Aaron, Zsembik, Leo, Yoshida, Takayuki, O’Malley, John J., Paletzki, Ronald F., Lieberman, Abby G., Nonaka, Mio, Deisseroth, Karl, Gerfen, Charles R., Penzo, Mario A., Kash, Thomas L., Holmes, Andrew
• Format: Article
• Language: English
• Subjects: 631/378/116/2394; 631/378/1457/1945; 631/378/1595/2636; 631/378/2645/1458; 64/60; Brain; Calcium; Calcium imaging; Fear; Freezing; Genetics; Hippocampus; Humanities and Social Sciences; Information processing; Learning; Mesencephalon; multidisciplinary; Neural networks; Neuroimaging; Neurons; Optics; Prefrontal cortex; Science; Science (multidisciplinary); Threats; Tonic immobility
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/s41586-023-07008-1

Animals can learn about sources of danger while minimizing their own risk by observing how others respond to threats. However, the distinct neural mechanisms by which threats are learned through social observation (known as observational fear learning 1 – 4 (OFL)) to generate behavioural responses specific to such threats remain poorly understood. The dorsomedial prefrontal cortex (dmPFC) performs several key functions that may underlie OFL, including processing of social information and disambiguation of threat cues 5 – 11 . Here we show that dmPFC is recruited and required for OFL in mice. Using cellular-resolution microendoscopic calcium imaging, we demonstrate that dmPFC neurons code for observational fear and do so in a manner that is distinct from direct experience. We find that dmPFC neuronal activity predicts upcoming switches between freezing and moving state elicited by threat. By combining neuronal circuit mapping, calcium imaging, electrophysiological recordings and optogenetics, we show that dmPFC projections to the midbrain periaqueductal grey (PAG) constrain observer freezing, and that amygdalar and hippocampal inputs to dmPFC opposingly modulate observer freezing. Together our findings reveal that dmPFC neurons compute a distinct code for observational fear and coordinate long-range neural circuits to select behavioural responses. Studies in mice show that observational fear learning is encoded by neurons in the dorsomedial prefrontal cortex in a manner that is distinct from the encoding of fear learned by direct experience.