Developmental energetics in the oviparous corn snake, Pantherophis guttatus, confirms a conservative evolutionary pattern in snakes

• Published in: Comparative biochemistry and physiology. Part A, Molecular & integrative physiology Molecular & integrative physiology, 2024-05, Vol.291, p.111592-111592, Article 111592
• Main Authors: Gallardo, Celeste R., Stewart, James R., Bidwell, Joseph R.
• Format: Article
• Language: English
• Subjects: Animals; Colubridae; Development; Egg incubation; Embryo, Nonmammalian - physiology; Female; Oviparity - physiology; Oxygen consumption; Pantherophis guttatus; Reptile; Snakes; Zea mays
• ISSN: 1095-6433; 1531-4332
• DOI: 10.1016/j.cbpa.2024.111592

Eggs of oviparous reptiles are ideal models for studying evolutionary patterns of embryonic metabolism since they allow tracking of energy allocation during development. Analyzing oxygen consumption of whole eggs throughout development indicates three patterns among reptiles. Embryos initially grow and consume oxygen exponentially, but oxygen consumption slows, or drops before hatching in some species. Turtles, crocodilians, and most lizards follow curves with initial exponential increases followed by declines, whereas embryonic snakes that have been studied exhibit a consistently exponential pattern. This study measured oxygen consumption of corn snake, Pantherophis guttatus, embryos to determine if this species also exhibits an exponential increase in oxygen consumption. Individual eggs, sampled weekly from oviposition to hatching, were placed in respirometry chambers for 24-h during which oxygen consumption was recorded. Embryos were staged and carcasses and yolk were weighed separately. Results indicate steady inclines in oxygen consumption during early stages of development, with a rapid increase prior to hatching. The findings support the hypothesis that embryonic oxygen consumption of snakes differs from most other non-avian reptiles. Total energy required for development was determined based on calorimetry of initial yolk compared to hatchlings and residual yolk and by integration of the area under the curve plotting oxygen consumption versus age of embryos. The cost of development estimates based on these two methods were 6.4 and 10.0 kJ, respectively. Our results emphasize the unique physiological aspects of snake embryogenesis and illustrate how the study of physiological characteristics can contribute to the broader understanding of reptilian evolution. [Display omitted] •P. guttatus embryos exhibited exponential oxygen consumption throughout development•Confirms prior findings of unique embryonic metabolism within the snake lineage•Cost of development estimated with bomb calorimetry and embryonic oxygen consumption•Developmental cost estimates were 6.38 Kj and 16.5 Kj, respectively