Temporal (Dis)Assembly of Peptide Nanostructures Dictated by Native Multistep Catalytic Transformations

• Published in: Nano letters 2024-02, Vol.24 (7), p.2250-2256
• Main Authors: Pal, Sumit, Saha, Bapan, Das, Dibyendu
• Format: Article
• Language: English
• Subjects: Biocatalysis; Catalysis; Hemin - chemistry; Nanostructures - chemistry; Peptides - chemistry
• ISSN: 1530-6984; 1530-6992
• DOI: 10.1021/acs.nanolett.3c04470

Emergence of complex catalytic machinery via simple building blocks under non-equilibrium conditions can contribute toward the system level understanding of the extant biocatalytic reaction network that fuels metabolism. Herein, we report temporal (dis)­assembly of peptide nanostructures in presence of a cofactor dictated by native multistep cascade transformations. The short peptide can form a dynamic covalent bond with the thermodynamically activated substrate and recruit cofactor hemin to access non-equilibrium catalytic nanostructures (positive feedback). The neighboring imidazole and hemin moieties in the assembled state rapidly converted the substrate to product(s) via a two-step cascade reaction (hydrolase-peroxidase like) that subsequently triggered the disassembly of the catalytic nanostructures (negative feedback). The feedback coupled reaction cycle involving intrinsic catalytic prowess of short peptides to realize the advanced trait of two-stage cascade degradation of a thermodynamically activated substrate foreshadows the complex non-equilibrium protometabolic networks that might have preceded the chemical emergence of life.