CRISPR/Cas9-mediated мultiplexed multi-allelic mutagenesis of genes located on A, B and R subgenomes of hexaploid triticale

Key message The first-time generation of hexaploid triticale plants harbouring variable panels of novel mutations in gene families involved in starch biosynthesis has been achieved by the subgenome-independent multiplexed CRISPR/Cas9-mediated editing.

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Bibliographic Details
Published in:Plant cell reports 2024-03, Vol.43 (3), p.59-59, Article 59
Main Authors: Miroshnichenko, Dmitry, Timerbaev, Vadim, Divashuk, Mikhail, Pushin, Alexander, Alekseeva, Valeria, Kroupin, Pavel, Bazhenov, Mikhail, Samarina, Mariya, Ermolaev, Aleksey, Karlov, Gennady, Dolgov, Sergey
Format: Article
Language:English
Subjects:
Agricultural biotechnology
Biomedical and Life Sciences
Biosynthesis
Biotechnology
Cell Biology
Conflicts of interest
CRISPR
CRISPR-Cas Systems - genetics
Efficiency
Focus Article
Gene families
Gene loci
Genomes
Genotype & phenotype
Iodine
Life Sciences
Mutagenesis
Mutagenesis - genetics
Mutation
Plant Biochemistry
Plant Sciences
Seeds
Transgenic plants
Triticale
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Description
Summary:Key message The first-time generation of hexaploid triticale plants harbouring variable panels of novel mutations in gene families involved in starch biosynthesis has been achieved by the subgenome-independent multiplexed CRISPR/Cas9-mediated editing.
ISSN:0721-7714
1432-203X
DOI:10.1007/s00299-023-03139-x