Effect of Anticancer Drug on the Cell Sensitivity to Ultrasound In Vitro and In Vivo

• Main Authors: Kamaev, Pavel P, Rapoport, Natalya Y
• Format: Conference Proceeding
• Language: English
• ISSN: 0094-243X
• DOI: 10.1063/1.2205533

Ultrasound bioeffects: loss of cell viability, cell membrane damage, intracellular uptake of anthracyclin drug doxorubicin (DOX), and cell proliferation, were investigated for drug-sensitive and multi-drug resistant (MDR) ovarian carcinoma cells. Cells were exposed to 1.1 MHz focused and 1.0 MHz unfocused ultrasound at different acoustic energies (1-1000 J/cm2) and DOX concentrations (1-20 mug/ml). It was found that MDR cells were more susceptible to the ultrasound action than sensitive cells if sonicated in suspension. At the same delivered energy, cell membrane damage increased with increasing acoustic pressure. Proliferation rate of the viable cells with compromised membranes was lower than that of intact cells. No effect of ultrasound (peak pressures up to 5 MPa, 30 sec exposure time) on the tumor growth in mice and their survival rates were found in the absence of DOX, while a synergistic effect of drug and ultrasound was observed in DOX presence.