Genetic characterization of Toxoplasma gondii from human and chicken isolates from Argentina

This study aimed to determine the nPCR-RFLP genotypes of newly obtained T. gondii isolates from human congenital toxoplasmosis cases in Argentina and to determine their allelic profiles for virulence genes ROP18/ROP5. In addition, the ROP18/ROP5 profiles were also determined for previously character...

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Published in:Parasitology research (1987) 2024-02, Vol.123 (2), p.129-129, Article 129
Main Authors: Bernstein, Mariana, Rudzinski, Marcelo, Schneider, Vanesa, Messina, Matías, Gos, María Laura, Helman, Elisa, Dellarupe, Andrea, Unzaga, Juan Manuel, Venturini, María Cecilia, Moré, Gastón, Pardini, Lais
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Brief Report
Genotypes
Genotyping
Immunology
Medical Microbiology
Microbiology
Protozoa
Toxoplasmosis
Virulence
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Summary:This study aimed to determine the nPCR-RFLP genotypes of newly obtained T. gondii isolates from human congenital toxoplasmosis cases in Argentina and to determine their allelic profiles for virulence genes ROP18/ROP5. In addition, the ROP18/ROP5 profiles were also determined for previously characterized T. gondii samples. Isolation from congenital toxoplasmosis cases was carried out in mouse bioassay from two placentas (P1 and P2). Genotyping for the new human isolates was performed by nPCR-RFLP using 10 markers. The samples analyzed for ROP18/ROP5 included the two newly obtained isolates (from the congenital toxoplasmosis cases) and nine previously genotyped T. gondii DNA samples from humans and chickens. The results for P1 and P2 named as TgHm18-02Arg and TgHm19-01Arg showed ToxoDB genotypes #14 (non-archetypal) and #2 (clonal type III), respectively. Non-archetypal #14 has been isolated from human cases before in Argentina. However, this is the first report of T. gondii clonal type III in a human case in the country. The ROP18/ROP5 combination was detected in nine samples: 3/3 ( n  = 1), 4/3 ( n  = 4), 4/4 ( n  = 3), and 3–4/4 ( n  = 1). Notably, the 4/4 profile was identified for the first time and exclusively in T. gondii samples from Misiones province (which borders southern Brazil). Further studies are required to corroborate the regionalization of the ROP18/ROP5 profiles in Argentina.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-024-08142-z