Analysis of free cisplatin in microdialysates and plasma ultrafiltrate by liquid chromatography-tandem mass spectrometry

•Utilization of UPLC-MS/MS for quantifying cisplatin in biological samples, offering high sensitivity and specificity.•Development of a stabilizing solution to maintain the stability of cisplatin during sample collection, freezing and analysis.•Co-eluting internal standard, dichloro(ethylenediamine)...

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Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2024-03, Vol.1235, p.124040-124040, Article 124040
Main Authors: Schmedes, A.V., Harlev, C., Bue, M., Petersen, E.K., Bergmann, M.L., Petersen, L.K., Stilling, M.
Format: Article
Language:English
Subjects:
Cisplatin
Microdialysis
Pharmacokinetics
UPLC-MS/MS
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Summary:•Utilization of UPLC-MS/MS for quantifying cisplatin in biological samples, offering high sensitivity and specificity.•Development of a stabilizing solution to maintain the stability of cisplatin during sample collection, freezing and analysis.•Co-eluting internal standard, dichloro(ethylenediamine)platinum(II), allowing accurate correction for ion suppression.•Thorough sample stability investigation under diverse conditions to ensure sample integrity and data accuracy.•Compared intravenous microdialysis and plasma utrafiltrate for cisplatin quantification, revealing pharmacokinetic equivalence. Cisplatin is a potent cytotoxic agent used in the treatment of various malignancies and exerts its antitumor effect through malignant cell DNA damage and apoptosis induction. Evaluation of systemic delivery of cisplatin is important in optimization of cisplatin treatment. However, accurate quantification of systemic cisplatin is challenging due to its various forms in circulation. This study aimed to develop a sensitive (LOQ 
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2024.124040