Regulation of metal homoeostasis by two F-group bZIP transcription factors bZIP48 and bZIP50 in rice

Zinc (Zn) deficiency not only impairs plant growth and development but also has negative effects on human health. Rice (Oryza Sativa L.) is a staple food for over half of the global population, yet the regulation of Zn deficiency response in rice remains largely unknown. In this study, we provide ev...

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Published in:Plant, cell and environment cell and environment, 2024-05, Vol.47 (5), p.1852-1864
Main Authors: Qing, Tao, Xie, Tian-Ci, Zhu, Qiao-Yun, Lu, Hai-Ping, Liu, Jian-Xiang
Format: Article
Language:English
Subjects:
Amino acid substitution
Amino acids
Copper
Gene deletion
Genes
Heavy metals
Iron
Localization
Mutants
Mutation
Nutrient deficiency
Oryza sativa
Plant growth
Rice
Seeds
Transcription
Transcription activation
Transcription factors
Zinc
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Summary:Zinc (Zn) deficiency not only impairs plant growth and development but also has negative effects on human health. Rice (Oryza Sativa L.) is a staple food for over half of the global population, yet the regulation of Zn deficiency response in rice remains largely unknown. In this study, we provide evidence that two F-group bZIP transcription factors, OsbZIP48/50, play a crucial role in Zn deficiency response. Mutations in OsbZIP48/50 result in impaired growth and reduced Zn/Fe/Cu content under Zn deficiency conditions. The N-terminus of OsbZIP48/OsbZIP50 contains two Zn sensor motifs (ZSMs), deletion or mutation of these ZSMs leads to increased nuclear localization. Both OsbZIP48 and OsbZIP50 exhibit transcriptional activation activity, and the upregulation of 1117 genes involved in metal uptake and other processes by Zn deficiency is diminished in the OsbZIP48/50 double mutant. Both OsbZIP48 and OsbZIP50 bind to the promoter of OsZIP10 and activate the ZDRE cis-element. Amino acid substitution mutation of the ZSM domain of OsbZIP48 in OsbZIP50 mutant background increases the content of Zn/Fe/Cu in brown rice seeds and leaves. Therefore, this study demonstrates that OsbZIP48/50 play a crucial role in regulating metal homoeostasis and identifies their downstream genes involved in the Zn deficiency response in rice.
ISSN:0140-7791
1365-3040
DOI:10.1111/pce.14852